蛋白激酶C及其亚型在肺恶性肿瘤中变化的研究  

Rescarch in Alteration of Activity of PKC and Its Isoforms in Lung Malignant Tumors

作　　者：赵冬梅[1] 许秀珍[1] 薛海晖 岳云卿 杨或 宗志宏[1] 于秉治[1] 王宏达 

机构地区：[1]沈阳第一临床学院呼吸疾病研究所,新疆克拉玛依卫生学校生物化学教研室

出　　处：《中国医科大学学报》1996年第4期373-376,共4页Journal of China Medical University

摘　　要：对肺恶性肿瘤及正常肺组织中蛋白激酶Ｃ的活性进行了对照研究。发现肺恶性肿瘤组织胞浆中的ＰＫＣ活性明显升高，Ｐ＜０．０５；胞膜中仅肉瘤组织ＰＫＣ活性显著升高，而肺鳞癌、腺癌组织胞膜中ＰＫＣ活性与正常肺组织无明显差异。另外，利用Ｗｅｓｔｅｒｎｂｌｏｔ法对ＰＫＣ亚型的分析表明：肺恶性肿瘤及正常肺组织中至少含有ＰＫＣ－α及ＰＫＣｐ－β而不含ＰＫＣ－ε，且ＰＫＣ－α及ＰＫｃ－β在肺恶性肿瘤中的含量较正常有不同程度的增加，其中ＰＫＣ－α出现膜转移。说明ＰＫＣ在肺恶性肿瘤发生机制中可能起重要的调控作用。e compared PKC activity in lung malignant tumors with that in normal lung tissue.Signiflcantlyincreased PKC activity can be observed in the cytosolic fraction of lung malignant tumors,but in thernernbranous fraction,only sarcoma tissue showed greatly elevated PKC activity.While lung malignantadenorna and squamate cell cancer showed nearly the same level of PKC activity as normal one. Also weanalysed the isoforms of PKC with method of Western blotting and found that at least PKC-αand PKC-β existed in lung tissue. And the content of PKC-α,β in lung malignant tumors increased to differentextent,and PKC-α.exhibited membrane translocation。All these indicate t hat PKC plays an importantrole in tumorigenesis and cell transfomation.

关 键 词：蛋白激酶C C亚型 肺肿瘤 

分 类 号：R734.202[医药卫生—肿瘤]