Detection and Comparison of Pathogen of Virus Disease in Pumpkin by RT-PCR and IC-PCR  

作　　者：YANG Guohui ZHANG Zhongkai CUI Chongshi 

机构地区：[1]Heilongjiang Academy of Agricultural Science, Harbin Heilongjiang 150086, PRC [2]College of Horticulture, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC [3]Agricultural Biotechnological Key Laboratory of Yunnan Province, Kunming Yunnan 650223, PRC

出　　处：《Journal of Northeast Agricultural University(English Edition)》2006年第2期129-132,共4页东北农业大学学报（英文版）

基　　金：The Open Fundation Item of Agricultural Biotechnological Key Laboratory of Yunnan Province (2001B- 3)

摘　　要：Two kinds of methods RT-PCR and IC-PCR were used to detect pathogen of virus disease of pumpkin and the sensitivity of the two methods was compared. The results showed that PRSV-W and CMV were detected in diseased samples gathered in Yunnan Province, while WMV and CMV were detected in diseased samples gathered in Heilongjiang Province. The sensitivity of RT-PCR is higher than that of IC-PCR, but the effect of IC-PCR in the specialization of bonding reaction and requisition for experiment material is better than that of RT-PCR.Two kinds of methods RT-PCR and IC-PCR were used to detect pathogen of virus disease of pumpkin and the sensitivity of the two methods was compared. The results showed that PRSV-W and CMV were detected in diseased samples gathered in Yunnan Province, while WMV and CMV were detected in diseased samples gathered in Heilongjiang Province. The sensitivity of RT-PCR is higher than that of IC-PCR, but the effect of IC-PCR in the specialization of bonding reaction and requisition for experiment material is better than that of RT-PCR.

关 键 词：PUMPKIN virus disease RT-PCR IC-PCR 

分 类 号：S436.429[农业科学—农业昆虫与害虫防治]