神经病理性痛对大鼠脊髓水平GABA_B受体亚型蛋白表达的影响  

Effects of neuropathic pain on the expression of spinal GABA_B recetor subtypes on rats

作　　者：朱珊珊[1] 聂鑫[2] 严蓉[2] 曾因明[2] 

机构地区：[1]江苏省徐州市第三人民医院麻醉科,221005 [2]江苏省麻醉医学研究所江苏省麻醉学重点实验室,徐州市221002

出　　处：《国际麻醉学与复苏杂志》2006年第6期325-328,共4页International Journal of Anesthesiology and Resuscitation

基　　金：江苏省教育厅开放课题资助项目(NoKJS02054;NoKJS050002);江苏大学临床医学科技发展基金(JLY20050064)

摘　　要：Objective To evaluate the effect of neuropathic pain on the expression of GABAB receptor subtypes in spinal cord of rats with chronic constriction injury.Methods 36 rats were divided into Sham group and CCI group respectively.In CCI group,the left common sciatic nerve of anesthetized rats was exposed,four silk ligatures（30） were tied loosely proximal to the sciatica’s trifurcation.In Sham group,Sham surgery was undergone by exposing the left sciatic nerve without ligation.Hind paw thermal withdrawl latency（TWL） to thermal stimulus and mechanical withdrawl threshold（MWT） of mechanical stimulus were recorded before ligation or on 1 d,3 d,5 d,7 d,10 d,14 d,21 d,28 d after ligation respectively（n=8）.Expression of GABAB receptor subtypes were detected before ligation and 1 d,3 d,7 d,14 d,28 d after ligation respectively using bothe immunohistochemical assay（n=6） and Western blot technique（n=4）.Results There were no significant differences on the values of MWT and TWL before ligation between Sham group and CCI group.Compared with Sham group,the MWT and TWL of rats in CCI group significantly decreased after ligation（P<0.01,P<0.05） and continued to be decreased until 28 d after ligation.Both immunoreactive neurons and protein content of GABAB1a or GABAB2 subtypes increased on the 3rd day after ligation,then both decreased on the 7th day after ligation,and lasted for 28 dafter ligation.（P<0.01,P<0.05）.There were no changes of GABAB1b after ligation（P>0.05）.Conclusion There existed differences among expression of spinal GABAB receptor subtypes on rats with chronic constriction injury induced neuropathic pain.The expression of spinal GABAB1a and GABAB2 decreased while there were no changes of GABAB1b on neuropathic rats.Objective To evaluate the effect of neuropathic pain on the expression of GABAB receptor subtypes in spinal cord of rats with chronic constriction injury. Methods 36 rats were divided into Sham group and CCI group respectively. In CCI group, the left common sciatic nerve of anesthetized rats was exposed, four silk ligatures （3～0） were tied loosely proximal to the sciatica＇s trifurcation. In Sham group, Sham surgery was undergone by exposing the left sciatic nerve without ligation. Hind paw thermal withdrawl latency （TWL） to thermal stimulus and mechanical withdrawl threshold （MWT） of mechanical stimulus were recorded before ligation or on 1 d, 3 d, 5 d, 7 d, 10 d, 14 d, 21 d, 28 d after ligation respectively （n = 8）. Expression of GABAB receptor subtypes were detected before ligation and 1 d, 3 d, 7 d, 14 d, 28 d after ligation respectively using bothe immunohistochemical assay （n = 6） and Western blot technique （n = 4）. Results There were no significant differences on the values of MWT and TWL before ligation between Sham group and CCI group. Compared with Sham group, the MWT and TWL of rats in CCI group significantly decreased after ligation （P 〈 0.01, P 〈 0.05） and continued to be decreased until 28 d after ligation. Both immunoreactive neurons and protein content of GABAB1 a or GABAB2 subtypes increased on the 3rd day after ligation, then both decreased on the 7th day after ligation, and lasted for 28 dafter ligation. （P 〈 0.01, P 〈 0.05）. There were no changes of GABAB1b after ligation （P 〉 0.05）. Conclusion There existed differences among expression of spinal GABAB receptor subtypes on rats with chronic constriction injury induced neuropathic pain. The expression of spinal GABAB1a and GABAB2 decreased while there were no changes of GABAB1b on neuropathic rats.

关 键 词：GABAB受体 神经病理性痛 受体亚型 蛋白表达 脊髓水平 神经递质受体 GABA转运体 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]