盐酸戊乙奎醚预处理对大鼠脑缺血/再灌注损伤所致脑梗死体积和神经元凋亡的影响  

Effect of pretreatment with penehyclidine hydrochloride on cerebral infarction volume and neuronal apoptosis in murine models of cerebral ischemia-reperfusion injury

作　　者：纵雪梅[1] 庞涛[1] 许铁[1] 耿德勤[2] 梁高永[1] 燕宪亮[1] 

机构地区：[1]徐州医学院附属医院急救中心,221002 [2]徐州医学院附属医院神经内科,221002

出　　处：《国际麻醉学与复苏杂志》2006年第6期334-337,共4页International Journal of Anesthesiology and Resuscitation

基　　金：江苏省高校自然科学研究计划项目(项目编号04KJD320206)

摘　　要：Objective To investigate the effect of a selective muscarinic receptor antagonist(penehyclidine hydrochloride) on three vessel occlusion model of acute global brain ischemia-reperfusion in rats.Methods One hundred and forty-four SD rats were randomly divided into four groups:sham operated group,vehicle pretreated group(saline 1 ml,i.p.),scopolamine pretreated group(0.01 mg/kg,i.p.) and penehyclidine hydrochloride pretreated group(0.01 mg/kg,i.p.) with drugs injected 40 min before ischemia respectively.The ischemic duration was 10 min.H-E staining,TUNEL staining and immunohistochemical reactions of bax and bcl-2 were carried out at the time points of 2 h,12 h,24 h,3 d and 7 d after reperfusion.Some animals were subjected to TTC staining for assessment of infarction volume 4 d after reperfusion.Results As compared with the vehicle pretreated group,both penehyclidine hydrochloride pretreatment and scopolamine pretreatment accelerated and extended the expression of bcl-2(P<0.01) in the CA1 subfield of hippocampus,meanwhile,penehyclidine hydrochloride pretreatment lowered the expression of bax(P<0.05 or P<0.01).Both of the drug pretreatments increased the survival pyramidal cells,decreased the numbers of apoptotic neurons and attenuated the cerebral infarction volume(P<0.05 or P<0.01).There was a more significant improvement in penehyclidine hydrochloride pretreated group than in scopolamine pretreated group in the same doses(P<0.05).Conclusion Penehyclidine hydrochloride may provide neuroprotection in acute global ischemia-reperfusion injury rats by altering the bcl-2/bax ratio.Objective To investigate the effect of a selective muscarinic receptor antagonist （penehyclidine hydrochloride） on three vessel occlusion model of acute global brain ischemia-reperfusion in rats. Methods One hundred and forty-four SD rats were randomly divided into four groups：sham operated group, vehicle pretreated group （saline 1 ml, i. p. ）, scopolamine pretreated group （0.01 mg/kg, i, p. ） and penehyclidine hydrochloride pretreated group （0.01 mg/kg, i. p. ） with drugs injected 40 min before ischemia respectively. The ischemic duration was 10 min. H-E staining, TUNEL staining and immunohistochemical reactions of bax and bcl-2 were carried out at the time points of 2 h, 12 h, 24 h, 3 d and 7 d after reperfusion. Some animals were subjected to TTC staining for assessment of infarction volume 4 d after reperfusion. Results As compared with the vehicle pretreated group, both penehyclidine hydrochloride pretreatment and scopolamine pretreatment accelerated and extended the expression of bcl-2 （P 〈 0.01） in the CA1 subfield of hippocampus, meanwhile, penehyclidine hydrochloride pretreatment lowered the expression of bax （P 〈 0.05 or P 〈 0.01）. Both of the drug pretreatments increased the survival pyramidal cells, decreased the numbers of apoptotic neurons and attenuated the cerebral infarction volume （P 〈 0.05 or P 〈 0.01）. There was a more significant improvement in penehyclidine hydrechloride pretreated group than in scopolamine pretreated group in the same doses （P 〈 0.05）. Conclusion Penehyclidine hydrochloride may provide neuroprotection in acute global ischemia-reperfusion injury rats by altering the bcl-2/bax ratio.

关 键 词：缺血/再灌注损伤 盐酸戊乙奎醚 神经元凋亡 脑梗死体积 大鼠脑 预处理 胆碱能受体阻滞剂 Ach释放 

分 类 号：R743.3[医药卫生—神经病学与精神病学]