荧光-噻唑蓝法检测红色纳米硒的抗氧化活性  

Research of Antioxidation of Red Nanose by Fluorescence Spectroscopy and Methylthianolyldiphenyl-tetrazolium Bromide Assay

作　　者：童春义[1] 薛昌刚[1] 刘选明[1] 肖苏尧[1] 俞丹密[1] 刘巧玲[1] 唐冬英[1] 赵李剑[1] 

机构地区：[1]湖南大学生命科学与技术研究院化学生物传感与计量学国家重点实验室,长沙410082

出　　处：《分析化学》2006年第12期1752-1754,共3页Chinese Journal of Analytical Chemistry

基　　金：湖南省农业生物纳米技术重大专项资助项目(No.03NKY1001)

摘　　要：采用荧光法检测红色纳米硒的抗氧化性,并结合噻唑蓝法(MTT法)研究红色纳米硒抗氧化对细胞的作用。Fenton反应产生稳定羟自由基,罗丹明B为荧光指示剂,磷酸盐缓冲液中检测了纳米硒清除羟自由基的效果。再将纳米硒与肝细胞QSG7701共培养,检测了不同时期培养基的氧化还原状况,并用MTT实验检测作用后细胞生长情况。结果显示:纳米硒具有良好抗氧化作用,能有效改善培养基中的氧化还原环境,同时促进了细胞生长。The aim of this article is to detect antioxidation of red selenium nanoparticles （nanose） provided by our lab and to primary study its biology characters in celluar. Fenton reaction was used to produce hydroxyl free radical, Rhodamine B was used as fluorescence indicator, and the detection solution is phosphate salt buffer. The measurement system was set up to measure the effects of nanose scavenging hydroxyl free radical. And then nanose and liver cells （ QSG7701 ） were incubated simultaneously, and the oxidation and deoxidization status of the culture medium were measured, while methylthianolyldiphenyl-tetrazolium bromide （MTT） assay was used to detect the growth of cells. The results display that nanose show well antioxidation action, improve the oxidation and deoxidization status, and boost the growth of cells.

关 键 词：荧光法 罗丹明B 纳米硒 噻哗蓝 

分 类 号：Q503[生物学—生物化学]