还原型谷胱苷肽治疗帕金森病大鼠模型的实验研究  

Experimental study of reduced glutathione in treatment of Parkinson's disease rats

作　　者：罗蔚锋[1] 包仕尧[1] 刘春风[1] 霍红梅[1] 吴冠会[1] 韩旺[1] 张志琳[1] 

机构地区：[1]苏州大学附属第二医院神经科,215004

出　　处：《临床神经病学杂志》2006年第6期441-443,共3页Journal of Clinical Neurology

摘　　要：目的观察还原型谷胱苷肽（GSH）治疗帕金森病（PD）大鼠模型的疗效。方法35只SD大鼠采用内侧前脑束注射6一羟多巴胺（6-OHDA）制作偏侧PD模型。术后第6周根据阿朴吗啡旋转试验将模型鼠分为部分PD组和完全PD组，各组再随机分为GSH亚组和对照亚组，并分别给予GSH或生理盐水腹腔注射，每日1次，共4周。治疗后第2周、4周、6周、8周进行阿朴吗啡旋转试验。结果共有27只大鼠制模成功，13只为部分PD模型，14只为完全PD模型；部分PDGSH亚组治疗后第4周、6周、8周阿朴吗啡旋转试验每分钟旋转次数较治疗前及相应的对照亚组显著减少（P〈0．05～0.01）；完全PDGSH亚组较治疗前及相应的对照亚组无显著变化。结论GSH治疗6-OHDA所致的部分PD大鼠模型有效，对完全PD大鼠模型无效。Objective To examine the effect of treatment with reduced glutathione（GSH） in 6-OHDA induced rat models of Parkinson＇s disease （PD）. Methods 35 SD rats were received injection of 6-OHDA by medial forebrain bundle to make lateral PD models. According to rotation test induced by Apomorphine 6 weeks later, the model rats were divided into partial PD group and total PD group. Each group was further divided into reduced glutathione hormone （GSH） sub-group and control sub-group randomly. The sub-groups were treated intraperitoneally with GSH or normai saline every day for 4 weeks, respectively. The functional outcome of each group was measured using the Apemorphine induced rotation test at 2, 4, 6 and 8 weeks after treatment. Results Successful PD models were made in 27 of 35 rats, which included 13 partial PD models and 14 total PD models. The numbers of rotation per minute induced by Apemorphine at 4, 6 and 8 weeks after treatment with GSH in partial PD group were significantly lower than that before treatment and in the control sub-group （P 〈0. 05 ～0. 01 ）. But in total PD group, no significant difference of rotation number was found after treatment with GSH compared with before treatment and the control sub-group. Conclusions GSH may improve neurological outcome of 6-OHDA induced partial PD model rats. But it is no effective for total PD model rats.

关 键 词：帕金森病 还原型谷胱苷肽 旋转试验 

分 类 号：R742.5[医药卫生—神经病学与精神病学]