钙蛋白酶抑制剂calpeptin对大鼠海马脑片缺氧无糖损伤的保护作用  

Protective effects of calpain inhibitor calpeptin on hypoxia/glucose deprivation injury in rat hippocampal slices

作　　者：匡重伸[1] 张育才[1] 曾因明[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,江苏徐州221002

出　　处：《中国药理学通报》2006年第12期1480-1483,共4页Chinese Pharmacological Bulletin

基　　金：江苏省教育厅省级重点实验室开放课题资助项目(NoKJS04004)

摘　　要：目的通过观察电生理学和神经元凋亡的变化,研究钙蛋白酶抑制剂calpeptin对大鼠离体海马脑片缺氧无糖损伤的保护作用。方法40片SD大鼠海马脑片随机分为两组:对照组和calpeptin组。根据人工脑脊液中calpeptin的浓度,再细分为1μmol.L-1组、10μmol.L-1组、100μmol.L-1组和200μmol.L-1组,每组8片脑片。采用细胞外记录技术观察calpeptin对大鼠离体海马脑片在缺氧无糖条件下顺向群峰电位(orthodromicpopulationspikes,OPS)及缺氧损伤电位(hypoxicinjurypotential,HIP)变化的影响,采用TUNEL法观察缺氧无糖损伤后海马脑片CA1区锥体细胞凋亡情况及calpeptin对它的影响。结果10μmol.L-1组、100μmol.L-1组和200μmol.L-1组HIP出现率及海马CA1区锥体细胞层凋亡细胞数减少,OPS恢复率和OPS恢复程度与对照组比较提高。而10μmol.L-1组、100μmol.L-1组和200μmol.L-1组之间各项指标差异无显著性。结论(10～200)μmol.L-1calpeptin可以减轻大鼠海马脑片的缺氧无糖损伤,其机制可能与calpeptin减少海马CA1区神经元凋亡有关。Aim To investigate effects of calpain inhibitor calpeptin against hypoxia/glucose deprivation injury in rat hippocampal slices. Methods Forty hippocampal slices were randomly divided into two groups： Control group and calpeptin group. Calpeptin group then divided again into 1 μmol·L^-1 group, 10 μmol·L^-1 group, 100 μmol·L^-1 group and 200 μmol·L^-1 group according to the concentration of calpeptin in ACSFOGD （ n = 8 per group）. By using electrophysiology method, changes of OPS and HIP during hypoxia/glucose deprivation process and effects of different concentration of calpeptin on it were observed. We also observed the neuronal apoptosis in hippocampal CA1 region after hypoxia/glucose deprivation and effects of different concentration of calpeptin on it. Restilts The HIP appearance rate and neuronal apoptosis in hippocampal CA1 region of 10 μmol·L^-1 group, 100 μmol·L^-1 group and 200μmol·L^-1group significantly reduced, the OPS recovery amplitude and OPS recovery rate significantly increased, and every index among 10μmol·L^-1 group, 100μmol·L^-1 group and 200 μmol·L^-1 group had no significant difference. Conclusion （ 10 - 200）μmol·L^-1 calpeptin improved the hypoxia/glucose deprivation induced brain injury of rat hippocampal slices and the mechanism might be related to the inhibition of the neuronal apoptosis by calpeptin.

关 键 词：钙蛋白酶 缺氧无糖损伤 海马脑片 顺向群峰电位 缺氧损伤电位 

分 类 号：R-332[医药卫生] R322.81