柴胡皂甙d对肝癌SMMC-7721细胞增殖的抑制作用  

Inhibitory effect of saikosaponins-d on human hepatocellular carcinoma cell line SMMC-7721 proliferation

作　　者：王艳丽[1] 和水祥[1] 罗金燕[2] 徐俊丽[1] 赵刚[1] 符寒[1] 

机构地区：[1]西安交通大学医学院第一附属医院消化内科,陕西西安710061 [2]西安交通大学医学院第二附属医院消化内科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2006年第6期572-574,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30271622)

摘　　要：目的观察柴胡皂甙d(SSd)对肝癌SMMC-7721细胞增殖的影响及其作用机制。方法肝癌SMMC-7721细胞株经内毒素(脂多糖,LPS)诱导,加入不同质量浓度的SSd作用后,MTT比色法检测细胞增殖抑制率,光学、电子显微镜和流式细胞仪观察细胞凋亡,放免法检测细胞前列腺素E2(PGE2)产生量。结果SSd对肝癌细胞增殖有明显的抑制作用,并呈剂量和时间依赖性。镜下观察,SSd作用组细胞可见典型凋亡小体形成,细胞凋亡率达17.5%。肿瘤细胞前列腺素E2的产生量,SSd作用组明显下降,接近环氧合酶2(COX-2)抑制剂尼美舒利(Nimesulide)作用组水平。结论SSd可能通过下调COX-2的表达,抑制PGE2产生而发挥抗癌作用。Objective To observe the effect of saikosaponins-d on proliferation of human hcpatoccllular carcinoma cell line SMMC-7721. Methods The human hepatocellular carcinoma cell line SMMC-7721 was cultured and treated with saikosaponins-d for 48 h. Cell growth rate was measured by monotetrazdium （MTT）, and the cell apoptosis was observed by transmission electron microscopy （TEM） and flow cytometry. The production of prostaglandin E2 （PGE2） in medium was measured by radioimmunoassay. Results The saikosaponins-d inhibited the proliferation and induced apoptosis of SMMC-7721 cells in a dose-and time-dependent manner. The production of PGE2 was obviously lower in saikosaponins-d treated group than that in control group, which was close to the level in nimesulide-treated group. Microscopic observation showed that typical apoptotic corpuscles formed in saikosaponins-d treated group, the apoptosis rate being 17.5%. Conclusion The saikosaponins-d inhibited the proliferation and induced apoptosis of the SMMC-7721 cells by the mechanism of inhibiting PGE2 production.

关 键 词：柴胡皂甙D 前列腺素E2 肝癌细胞 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]