DNA甲基化研究方法的回顾与评价  

Review and evaluation of methodology of research on DNA methylation

作　　者：顾婷婷[1] 张忠明[1] 郑鹏生[1] 

机构地区：[1]西安交通大学医学院第一附属医院妇产科

出　　处：《中国妇幼健康研究》2006年第6期555-560,F0003,共7页Chinese Journal of Woman and Child Health Research

摘　　要：DNA甲基化是表观遗传学的重要组成部分,在维持正常细胞功能、遗传印记、胚胎发育以及人类肿瘤发生中起着重要作用,是目前新的研究热点之一。随着对甲基化研究的不断深入,各种各样甲基化检测方法被开发出来以满足不同类型研究的要求,这些方法概括起来可分为3类:基因组整体水平的甲基化检测、基因特异位点甲基化的检测和新甲基化位点的寻找。该文主要介绍目前应用的大部分DNA甲基化研究方法,并对其相关特性进行简要分析与总结。DNA methylation, a very important component in epigenetics, plays important roles in maintaining normal cellular functions, gent imprinting, embryonic development, and human tumorigenesis, and now it becomes one of the hot points of research. With progresses in study on methylation, sorts of detecting techniques of methylation have been developed to meet requirements of various researches. These research methods can be classified into three groups： genome-wide methylation analysis, gene-spocific methylation analysis and newly methylated sites screening. In this article, we introduced existing DNA methlylation detecting techniques and summarized their own characteristics at the same time.

关 键 词：表观遗传学 DNA甲基化 甲基化研究方法 重亚硫酸盐 

分 类 号：R394[医药卫生—医学遗传学]