水貂源犬瘟热病毒的分离鉴定  

Isolation and RT-PCR Identification of Canine Distemper Virus from Mink

作　　者：苏凤艳[1] 宗春苗[1] 王卓聪[1] 丁庆东[1] 王全凯[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《吉林农业大学学报》2006年第6期674-677,共4页Journal of Jilin Agricultural University

基　　金：国家"948"项目(2001-364)

摘　　要：利用Vero传代细胞从病死水貂肝脏中分离获得1株病毒。该毒株可使培养细胞出现明显的细胞病变(CPE)。以提取病毒感染细胞总RNA为模板进行反转录聚合酶链式反应(RT-PCR),结果扩增出的基因片段大小与预期值相符。根据病毒的致细胞病变特征和RT-PCR鉴定结果,确证该毒株为犬瘟热病毒。A mink-originated strain of canine distemper virus was isolated in Vero cell from a mink liver. CDVSJ strain exhibited a typical vacuous degeneration. A fragment of 843 bp was amplified from Vero cell incubated with the isolated virus strain by reverse transcriptase polymerase chain reaction（RT-PCR） . From the results described above, it can be concluded that the strain is a new strain of mink-originated CDV.

关 键 词：水貂 犬瘟热病毒 分离鉴定 RT-PCR 

分 类 号：S852[农业科学—基础兽医学]