蛇毒降纤酶对兔眼外伤性PVR纤维连接蛋白、波形蛋白的影响  

The effect of Defibrase on fibronectin and vitronectin in traumatic rabbit eyes with proliferative vitreoretinopathy

作　　者：招志毅[1] 区显宁[2] 段芳[2] 

机构地区：[1]桂林医学院附属医院眼科,广西桂林541001 [2]广西医科大学第一附属医院眼科,广西南宁530021

出　　处：《华夏医学》2006年第6期1063-1065,共3页Acta Medicinae Sinica

摘　　要：目的探讨蛇毒降纤酶对外伤性增生性玻璃体视网膜病变(PVR)玻璃体中纤维连接蛋白(fibronectin,FN)、波形蛋白(vitronectin,VN)的影响。方法对20只兔子采用兔眼后节穿通伤加注血法或/和加蛇毒降纤酶制备实验模型,随机分成正常对照组(20眼)、全血组(10眼)、蛇毒降纤酶组(10眼)。利用免疫组化SP法观察实验各组,检测PVR玻璃体腔增殖膜中FN、VN的含量。结果蛇毒降纤酶组增殖膜中FN、VN阳性面积比值分别为0.7323±0.1869、0.4635±0.1071;全血组FN、VN阳性面积比值分别为4.6211±1.1502、1.0289±0.2654。蛇毒降纤酶组增殖膜中FN、VN的阳性表达均低于全血组(P<0.01)。结论蛇毒降纤酶能够降低PVR增殖膜中FN、VN的阳性表达,抑制PVR的发生、发展。Objective： To study the effect of Defibrase on fibronectin and vitronectin in experimental traumatic proliferative vitreoretinopathy. Methods：Twenty experimental models were induced by posterior penetrating eye injury and vitreous infusing blood or/and Defibrase in the rabbit. These rabbits were divided into three groups randomly： normal control group （twenty eyes）, full blood group （ten eyes）, and defibrase group （ten eyes）. The positive expression of FN, VN in the proliferative membranes were examined by the immunohistochemical SP. Results ：Compared with the full blood group, the positive expression of FN,VN in the proliferative membranes were decreased in the Defibrase group （P〈0. 01）. Conclusion： Defibrase can decrease the positive expression of FN,VN in the proliferative membranes,inhibit the development of PVR.

关 键 词：蛇毒降纤酶 增生性玻璃体视网膜病变 纤维连接蛋白 

分 类 号：Q959.836[生物学—动物学] R779.1[医药卫生—眼科]