视神经损伤后Rock在大鼠视网膜中的分布及表达研究  

Distribution and expression of Rock in rat retina after optic nerve crushed

作　　者：王艳华[1] 王东武 吴楠[1] 刘东宁[1] 王一[1] 

机构地区：[1]第三军医大学西南医院眼科,重庆400038 [2]山西省晋中市武警8650部队医院,030600

出　　处：《重庆医学》2006年第24期2231-2232,共2页Chongqing medicine

基　　金：全军医药卫生科研基金资助(01Z039)

摘　　要：目的观察视神经损伤后Rock在视网膜中的分布及表达变化。方法取36只成年Long Evans大鼠为研究对象,分为正常,视神经损伤1、3及7d组共4组,每组9只,通过免疫组化染色,研究Rock在视网膜中的分布变化；western blot分析统计Rock表达变化。结果正常及视神经损伤后1d,Rock主要分布于视网膜神经节细胞(retinal ganglion cells,RGCs)层；3d分布于RGCs及内丛状层；7d分布于RGCs、内丛状层、内核层及外丛状层。western blot示：视神经损伤后3、7d,Rock表达均高于正常组(P＜O．01～0．05)。但7d表达量最高。结论视神经损伤可显著增加Rock在视网膜中的表达量及分布范围,它在视神经损伤后再生过程中发挥重要的作用。Objective To study the distribution and expression of Rock in the rat retina after optie nerve crushed. Methods Ex periments were performed on 36 adult Long Evans rats. There were four experiment groups： normal group, 1d ,3d and 7d after optic nerve crushed groups. And every group had 9 rats. The expression of Rock was analyzed by Western blot analysis, and the dis tribution was assayed by immunohistochemistry. Results In normal retina and the retina after 1d of optic nerve crushed, Rock distributed in RGCs layer. In the retinal after 3d of optic nerve crushed, it existed in RGCs and inner plexiform layer. However, its immunoreaetivity was abundant not only in RGCs and inner plexiform layers but also in inner nuclear and outer plexiform layers after 7d of optic nerve crushed. Western blot analysis showed the expression of Rock after 3d,7d of optic nerve crushed increased signifieantly than that of normal retina（P〈0. 01-0. 05）. Conclusion The distribution and expression of Rock were extended and enhaneed after optic nerve crushed.

关 键 词：视神经损伤 蛋白质丝氨酸苏氨酸激酶 视网膜 

分 类 号：R779.1[医药卫生—眼科]