AS-PCR在牛羊异种核移植上的应用  

Application of AS-PCR in interspecies transplantation between goat and cattle

作　　者：戴津泼[1] 华松[1] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2006年第11期1-4,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"高科技项目(2001AA213081)

摘　　要：利用PCR反应延伸过程中,3′端碱基不配对,PCR反应不能启动的等位基因特异性PCR分析法(AS-PCR)原理,采用生物软件DNASTAR分析牛、羊线粒体细胞色素b(Cytb)基因,以牛、羊碱基差异较大位点作为上游引物的3′端来设计引物,进行PCR扩增反应,分析牛羊异种克隆胚中线粒体的变化情况。结果表明,AS-PCR法是一种快速、有效鉴定异种重构胚中线粒体异质性的方法。In the PCR elongation process,the reaction can＇t start when 3＇-end-base doesn＇t match. To study the heteroplasmy of mitochondria ,bio-assay software DNASTAR was used to align Cytb gene of cattle and goat. Then PCR primers were designed with different bases between goat and cattle with 3＇-endbase as upstream primers. The change of mitochondria in interspecies goat-cattle nuclear transfered embryos was identified by AS-PCR. The results indicate that AS PCR is a rapid and efficient method to identificate mitochondria heteroplasmy in interspecies reconstructed embryos.

关 键 词：AS-PCR 核移植 线粒体 细胞色素B 

分 类 号：S852.23[农业科学—基础兽医学]