青龙衣多糖对H_(22)型肿瘤细胞的ATPase活性影响的研究  

Research on effect of ATPase in tumor cells of H22 type with Qinglongyi Polysaccharide

作　　者：季宇彬[1] 陈海继[1] 汲晨锋[1] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研发中心,哈尔滨150076

出　　处：《药品评价》2006年第6期412-415,共4页Drug Evaluation

摘　　要：目的研究青龙衣多糖对H22型肿瘤细胞的ATPase活性的影响。方法按照测定无机磷含量的方法对H22肿瘤细胞的Ca2+-ATPase、Na+K+-ATPase和Mg2+-ATPase活性进行了测定。结果发现青龙衣多糖可以降低ATPase的活性,除青龙衣多糖中、高剂量组显著抑制Ca2+-ATPase活性,高、中、低剂量组都显著降低Na+-K+-ATPase和Mg2+-ATPase活性(P<0.05)。结论ATPase活性降低导致了膜电位下降,细胞容积降低,可能引起细胞凋亡发生,Ca2+调节凋亡发生,Mg2+调控ATPase的活性,可能是青龙衣多糖抗肿瘤的机制之一。Objective To study the effect of ATPase in tumor cells of H22 type with Qinglongyi Polysaccharide. Method ATPase test kits are used to determine the activities of Ca^2＋-ATPase, Na^＋K ^＋ATPase and Mg^2＋-ATPase in tumor cells of H22 type. Results It showed Qinglongyi polysaccharide high-, middle-, low-dosage group could decrease the activity of Na^＋-K^＋-ATPase and Mg^2＋-ATPase besides the high-, middle- dosage group could inhibit the activity of Ca^2＋- ATPase （P〈0.05）. Conclusion The decrease of ATPase makes the membrane potential and the cell volume reduce they lead to apoptosis. Ca2^2＋ can regulate apoptosis, and Mg^2＋ regulate the activity of ATPase. So the Qinglongyi Polysaccharide may kill the tumor by the decrease of ATPase.

关 键 词：青龙衣多糖 离子通道 ATPASE活性 细胞凋亡 

分 类 号：R73-3[医药卫生—肿瘤]