新型CpG ODN增强乙肝疫苗诱导IgG2a类抗体产生的实验研究  

Study of New CpG ODNs on enhancement of IgG2a production induced by hepatitis B vaccine

作　　者：丛中一[1] 包木胜[1] 万敏[1] 王莉[1] 李大鹏[1] 王丽颖[1] 于永利[1] 

机构地区：[1]吉林大学基础医学院免疫学教研室,分子生物学教研室,长春130021

出　　处：《中国免疫学杂志》2006年第11期987-990,996,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金海外杰出青年基金资助(30328010)

摘　　要：目的寻找能增强乙肝疫苗刺激IgG2a类抗体产生,使机体处于Th1样免疫环境的新型乙肝疫苗佐剂。方法选用自行设计的A、B、C型CpGODN,并以发表的A、B型CpGODN作为阳性对照,与重组乙肝疫苗混合后于第0、4周免疫BALB/c小鼠,用ELISA法检测免疫小鼠血清中HBsAb水平及种类。结果各型CpGODN都能增强乙肝疫苗刺激HBsAb的产生水平,CpGODN+乙肝疫苗免疫的小鼠血清中HBsAb类型为IgG2a>>IgG1,而单独应用商品化重组乙型肝炎疫苗的小鼠血清中HBsAb类型为IgG1>>IgG2a。结论各型CpGODN对重组乙型肝炎疫苗[含Al(OH)3佐剂]均具有增效作用,而且可以诱导机体产生倾向于Th1途径的免疫应答反应。Objective：To search for new adjuvant for hepatitis B vaccine that could stimulate the production of IgG2a and induce Th1-like immune circumstance. Methods.BALB/c mice were immunized with recombinant hepatitis B vaccine only or combined with CpG ODNs designed by our lab（ including type A, B and C） and CpG ODNs published in articles at 0th and 4th week. The HBsAb level and the IgG subsets in the serum of mice were measured by ELISA. Results：All types of CpG ODN could enhance the production of HBsAb stimulated by hepatitis B vaccine. The level of IgG subsets of mice immunized with hepatitis B vaecine combined with CpG ODN was IgG2a 〉 〉 IgG1 , and that of mice immunized with hepatitis B vaccine only was IgG1〉〉IgG2a. Conclusion：All types of CpG ODN can enhance the effect of recombinant hepatitis B vaccine and induce Th1-like immune response.

关 键 词：CPG ODN 乙型肝炎疫苗 佐剂 IGG2A 

分 类 号：R392.11[医药卫生—免疫学]