光照对中国对虾稚虾3种消化酶活力的影响  

Effects of light on specific activities of three digestive enzymes in juvenile Chinese shrimp Fenneropenaeus chinensis

作　　者：王芳[1] 宋传民[2] 丁森[1] 董双林[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003 [2]山东省东营市河口区海洋与渔业局,山东东营257200

出　　处：《中国水产科学》2006年第6期1028-1032,共5页Journal of Fishery Sciences of China

基　　金：国家自然科学基金资助项目(30571441)

摘　　要：在实验室条件下研究光照(光照周期、光照强度和光色)对中国对虾(Fenneropenaeus chinensis)稚虾3种消化酶(蛋白酶、淀粉酶和脂肪酶)活力的影响。在本实验条件下,主要结果如下:(1)4种光照周期下,对虾3种消化酶的活力差异不显著(P>0.05);(2)在完全黑暗、6μmol.m-2.s-1、30μmol.m-2.s-1和110μmol.m-2.s-1光照强度下,对虾蛋白酶的活力分别为0.361 U.mg-1(protein)、0.081 U.mg-1(protein)、0.088 U.mg-1(protein)和0.273 U.mg-1(protein),完全黑暗和110μmol.m-2.s-1下的活力显著高于其他两个光照强度(P<0.05),且两者间的差异达到显著水平(P<0.05);中国对虾淀粉酶的活力分别为0.386 U.mg-1(protein)、0.095 U.mg-1(protein)、0.111 U.mg-1(protein)和0.315 U.mg-1(protein),完全黑暗和110μmol.m-2.s-1条件下的活力显著高于其他两个光照强度(P<0.05),两者间的差异亦达到显著水平(P<0.05);对虾脂肪酶的活力分别为0.147×10-2U.mg-1(protein)、0.150×10-2U.mg-1(protein)、0.170×10-2U.mg-1(protein)和0.183×10-2U.mg-1(protein),完全黑暗和110μmol.m-2.s-1条件下的活力差异达到显著水平(P<0.05);(3)不同光色下,对虾蛋白酶和淀粉酶的活力差异不显著(P>0.05),脂肪酶的活力则存在一定的差异,蓝光和黄光下,对虾脂肪酶的活力分别为0.093×10-2U.mg-1(protein)和0.107×10-2U.mg-1(protein),显著高于白光和绿光下的活力(P<0.05),但蓝光组和黄光组间的差异不显著(P>0.05)。The effects of light（photoperiod, light intensity and light color） on specific activities of three digestive enzymes（protease, amylase and lipase） in juvenile Chinese shrimp Fenneropenaeus chinesis were studied under laboratory conditions. The main results were as follows： 1, Photoperiod did not influence the specific activities of protease, amylase and lipase significantly（P 〉0.05）. 2, The values of specific activity of protease were 0. 361 U· mg^- 1 （protein）, 0. 081 U· mg^- 1 （protein）, 0. 088U· mg^- 1 （protein） and 0.273 U· mg^- 1 （protein） under light intensities of 0μmol · m^- 2·s^- 1,6μmol · m^- 2·s^- 1,30μmol · m^- 2·s^- 1and 110μmol · m^- 2·s^- 1,respectively. The specific activities under light intensity of 0μmol · m^- 2·s^- 1 were significantly higher than that of 110μmol · m^- 2·s^- 1, and both were significantly higher than the other treatments（ P 〈 0.05）. The specific activities of amylase were 0. 386 U· mg^- 1 （protein）, 0.095 U· mg^- 1 protein）, 0.111 U· mg^- 1 （protein） and 0.315 U· mg^- 1 （protein） under the four light intensities （ from low to high）, respectively, and were similar to protease enzyme variation in pattern. The specific activities of lipase under the four light intensities（from low to high） were 0. 147 × 10^- 2 U· mg^- 1 （protein） ,0. 150 × 10^- 2 U· mg^- 1（protein）, 0.170 × 10^- 2 U· mg^- 1 （protein） and 0.183× 10^- 2 U· mg^- 1（protein）, respectively, and there was significant difference between 0μmol · m^- 2·s^- 1 and 110μmol · m^- 2·s^- 1 treatments（ P 〈 0.05）. 3. No significant difference was found in specific activities of protease nor amylase under different light color treatments（P 〉0.05）. Under blue and yellow lights, the specific activities of lipase were 0.093 × 10^- 2 U· mg^- 1 （protein） and × 10^- 2 U· mg^- 1 （protein）, respectively, and were significantly higher than those under white and green lights（P〈 0.05） ;no signifi

关 键 词：中国对虾 稚虾 消化酶活力 光照周期 光照强度 光色 

分 类 号：S968.2[农业科学—水产养殖]