Lactobacillus diolivorans二醇脱水酶的分离纯化与酶学性质  

Purification and characterization of diol dehydratase of Lactobacillus diolivorans

作　　者：孟晓蕾[1,2] 齐向辉[1,2] 韦宇拓[1,2] 黄日波[1,2] 

机构地区：[1]广西大学广西亚热带生物资源保护利用重点实验室,广西南宁530004 [2]南宁中诺生物工程公司,广西南宁530003

出　　处：《化工学报》2006年第12期2938-2942,共5页CIESC Journal

基　　金：国家高技术研究发展计划项目(2003AA001039)~~

摘　　要：1，3-丙二醇（1，3-propanediol，1，3-PD）是一种重要的化工和医药中间体原料，是良好的溶剂、抗冻剂或保护剂，是聚酯——聚对苯二甲酸丙二醇酯（PTT）和聚氨酯的单体，可在地毯、纺织、工程塑料等领域中广泛应用，也可用于食品、化妆品和制药等行业食品．当前化工合成是生产1，3-丙二醇的主要方法，Diol dehydratase of Lactobacillus diolivorans was purified by ammonium sulfate precipitation and gel filtration chromatography. The molecular mass of the native complex was estimated to be 200× 10^3 by nondenaturing polyacrylamide gel electrophoresis. Three main bands were present after SDS-PAGE with molecular masses of 62 × 10^3 , 35 × 10^3 and 22×10^3 , respectively. Km for the three main substrates were 1.3 mmol· L^-1 for 1,2-propanediol, 5.8 mmol ·L^-1 for glycerol and 10.3 mmol·L^-1 for 1, 2- ethanediol. Km for adenosylcobalamin coenzyme was 1.5 μmol·L^-1, which was required for the enzyme catalysis. The optimal reaction conditions of the enzyme were pH 8. 6 and 37℃.

关 键 词：LACTOBACILLUS diolivorans 二醇脱水酶 辅酶B12 甘油脱水酶 

分 类 号：Q55[生物学—生物化学]