一种具有光致变色特性的膜蛋白/聚合物复合膜  

A MEMBRANE PROTEIN/POLYMER COMPOSITE FILM WITH PHOTOCHROMIC RESPONSE

作　　者：马德旺[1] 明明[1] 洪洁[2] 吴佳[1] 李庆国[3] 黄伟达[2] 丁建东[1] 

机构地区：[1]聚合物分子工程教育部重点实验室复旦大学高分子科学系,上海200433 [2]复旦大学生命科学院生物化学系,上海200433 [3]复旦大学生命科学院生理学与生物物理学系,上海200433

出　　处：《高分子学报》2006年第9期1078-1082,共5页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号20374015;20574013;50533010);教育部科学技术研究重大项目(项目号305004);国家重点基础研究发展规划项目(973)(项目号2005CB522700);上海市科技发展基金(基金号04JC14019;055207082)资助项目

摘　　要：通过基因工程技术将古紫质4(archaerhodopsin-4,AR4)膜蛋白的基因转入本身不表达该蛋白质、也不表达玉红素的嗜盐菌株L33中,去除了天然菌株xz515所获得的AR4膜蛋白脂环境中的玉红素,优化了AR4的光致变色性能.将重组古紫质4包埋于聚乙烯醇中制成复合膜,并通过提高体系的pH值,延长了M态的寿命,进一步实现了简单图案记录,验证了该活性蛋白质与聚合物的复合材料具有光信息存储功能.A gene of a membrane protein, Archaerhodopsin-4 （AR4）, was expressed into an H. salinarum strain L133,which is negative for synthesis of bacterio-opsin and whose lipid environment is free of carotenoid. The carotenoid has three characterized absorption peaks at 480,508 and 544 nm which cover the absorption peak of membrane protein AR4 at 5700 nm at the ground state, in contrast to the wide-type AR4 isolated from the conventional strain H. sp. xz515 ,the recombinant AR4 removed the binded carotenoid .The underlying photochromic properties of the recombinant AR4 was thus improved, and the contrast between M410 intermediate and ground state was greatly enhanced. Composite films were further made by embedding the recombinant AR4 into poly（vinyl alcohol） （PVA）. By enhancing its pH, the decay of the M intermediate was slowed down. A simple pattern recording as an optical information storage material was also realized by the compostie films. During this process, light was illuminated into the composite covered with a mask, and the pattern in the mask was observed even after removing the mask. Hence, this paper demonstrates that the polymer technique and gene engineering could be combined to prepare information materials with both the biological activities of proteins and the good processing properties of polymers.

关 键 词：细菌视紫红质 古紫质 聚乙烯醇 信息存储 基因工程 

分 类 号：O631.3[理学—高分子化学]