全眼球冷冻保存角膜在穿透性角膜移植中的应用  

Clinical application of corneal grafts preserved by whole eyeball cryopreservation in penetrating keratoplasty

作　　者：李冰[1] 史静华[1] 韩玉萍[1] 

机构地区：[1]山西省眼科医院,太原030002

出　　处：《中国实用眼科杂志》2006年第12期1259-1260,共2页Chinese Journal of Practical Ophthalmology

基　　金：山西省科学技术发展计划项目资助(042028)

摘　　要：目的评价长期全眼球冷冻保存角膜行穿透角膜移植的临床效果。方法采用抽空房水,C3F8充填前房全眼球冷冻保存(-20℃)角膜行20例穿透角膜移植术,最短保存时间为28d,最长为133d,平均39.6d,本组20例患者,病灶范围均在8mm以上,穿孔达(4～5)mm左右。结果术后半年,角膜半透明5例,内皮细胞平均密度为1076.3celll/mm2,视力维持在数指/1尺,14例患者角膜混浊,视力手动～光感,其中5例患者半年后再次行穿透角膜移植术。术后并发症:1例患者在术后3m时,发生继发青光眼,患者坚决要求行眼球摘除术。结论前房注气(C3F8)全眼球冷冻保存角膜使角膜内皮细胞具有一定活性,方法简便、易行,可为眼科急诊提供穿透角膜材料。Objective To assess the clinical effect of penetrating keratoplasty with corneal grafts after long-term whole eyeball cryopreservation. Methods 20 cases of penetrating keratoplasty were programmed with grafts that were preverved by whole eyeball cryopreservation after injecting C3F8 into anterior chambers. The preserved term of these grafts was from 28 days to 133 days, average 39.6 days. The focal range of all these 20 cases were more than 8ram,and with 4-Smm perforation. Results 6 months later, the corneal grafts of 5 cases were translucent, and the average cell density of corneal endothelium was 1076.3cell/mm^2. 14 corneal grafts were turbid and the patients visual acuities were LP-HM, 5 of them had the second time penetrating keratoplasty after 6 months and achieved satisfied results. Complications of the operation： I patient got glaucoma even the corneal graft was transparent, and the patient asked for an exenteration of eyeball firmly. Conclusion Whole eyeball cryopreservation after C3F8 injection to prevere corneal grafts can keep some of the activity of the corneal endothelium, and the method was simple and easy, thus can provide penetrating grafts for ophthalmologic emergency.

关 键 词：全眼球冷冻 角膜 穿透角膜移植术 

分 类 号：R779.65[医药卫生—眼科]