色素上皮细胞衍生因子对脑胶质瘤抑制作用的研究  

The Inhibitory effect of pigment epithelium-derived factor on invasion of gliomas

作　　者：张弢[1] 关明[1] 吕元[1] 

机构地区：[1]复旦大学附属华山医院检验医学中心,上海200040

出　　处：《中华检验医学杂志》2006年第12期1093-1096,共4页Chinese Journal of Laboratory Medicine

基　　金：教育部博士点基金资助项目(20030246060);上海市青年科技启明星计划资助项目(04QMX1408);复旦大学世纪之星资助项目;上海市临床医学重点建设学科项目

摘　　要：目的评价重组色素上皮细胞衍生因子(rPEDF)对与胶质瘤有关的血管活性因子、增殖和侵袭的影响。方法采用酶联免疫吸附测定(ELISA)法检测血管内皮细胞生长因子(VEGF)与血小板反应素-1(TSP-1)的变化;四甲基偶氮唑蓝[3-(4,5-dimthy-2-2thiazoly)2,5-dipheny-2H-tetrazoliunbromide,MTT]法检测胶质瘤细胞增殖率;细胞侵袭实验检测rPEDF抑制胶质瘤细胞侵袭性。结果实验数据显示:A172PEDF组(将rPEDF加入胶质瘤细胞A172中)中VEGF表达量为0·29±0·03,与未加PEDF蛋白的胶质瘤细胞作对照组(A172con组,1·31±0·02)相比,减少4·4倍,(P<0·05),同时TSP-1的表达量A172PEBF组为1585±105·67,与A172con组的,351±20·65相比,表达上调4·5倍(P<0·01);侵袭实验结果表明:胶质瘤细胞穿透数为88·67±3·32明显多于rPEDF处理过的细胞(44·41±13·81,2·0倍),加入促血管生成因子—VEGF后,细胞穿透数仍减少至69·4%(69·42±6·35,P<0·05),表明rPEDF能有效抑制胶质瘤细胞的体外侵袭性,至少部分是在血管生成因子调节的作用下。结论rPEDF是抑制胶质瘤增殖和侵袭的主要因素。Objective To assess the impact of recombinant pigment epithelium-derlved factor （rPEDF） on the growth and invasion of the glioma cells. Methods The level of vascular enthothelial growth factor （VEGF） and thrombospondin-1 （TSP-1） were evaluated by enzyme-linked immunoadsorbent assay （ELISA） ; cell proliferation assays were performed by MTY assay; the cell migratory assay was used for evaluating its inhibitory migration rate. Results The VEGF expression （0. 29 ± 0. 03 ） of A172PEDF （ added the PEDF to the A172 cells） decreased 4. 4 times compared with the A172con （ not added the PEDF to the A172 cells, 1.31 ±0.02, P 〈0.05） as well as increased the TSP-1 （1 585 ± 105.67, 4.5 times, P 〈0.01） compared with the A172con （351 ±20. 65） ; the migratory cells of glioma（88, 67 ±3. 32）were 2 times higher than those of added rPEDF（44. 41 ± 13.81 ） ,and the migratory rate was 69.4% （ 69.42 ± 6. 35 ,P 〈 0. 05 ） even in the presence of VEGF. rPEDF could decrease the migratory capacity of the glioma cells at least in the presence of VEGF partly. Conclusions rPEDF is a potent factor for the inhibition proliferation and migration in gliomas in part by regulation of angiogenestic factors.

关 键 词：色素上皮细胞衍生因子 胶质瘤 增殖 侵袭 

分 类 号：R739.4[医药卫生—肿瘤]