影响小鼠冻融精子体外受精率的主要因素探讨  

Exploration of Factors Affecting in vitro Fertilization Rate of Post-Thawed Mouse Spermatozoa

作　　者：赵丹凤[1] 刘丽均[1] 狄敏[1] 赵立虎[1] 张春燕[1] 张守纯[2] 徐平[1] 

机构地区：[1]中国科学院上海实验动物中心,上海201615 [2]沈阳农业大学畜牧兽医学院,辽宁沈阳110161

出　　处：《中国比较医学杂志》2006年第12期736-739,743,共5页Chinese Journal of Comparative Medicine

基　　金：上海市科技发展基金项目(编号:044909003);上海市科技发展基金项目(编号:054909007)

摘　　要：目的分析影响小鼠精子冷冻成功的因素。方法用含3%脱脂奶和18%棉子糖的冷冻保护剂分别冷冻C57BL/6J、FVB/NJ、B6D2F1、KM和ICR小鼠精子。复苏后,评价小鼠精子的冷冻效果和受精能力。结果各品系小鼠精子冷冻前后的受精率不同;新鲜精子体外受精时,C57BL/6J、FVB/NJ、B6D2F1,KM和ICR五个品系的小鼠的受精率分别为81.4%、87.2%、90.4%、82.7%和79.4%而冻融精子的体外受精率分别为6.7%、46.0%、76.8%、59.2%和31.9%,差异极显著(P<0.01)。结论遗传背景不同的小鼠品系,精子的受精能力及对冷冻的耐受能力不同。Objective To investigate the factors affecting cryopreservation of mouse sperm. Methods Mice sperm from C57BL/6J, FVB/NJ, B6D2F1 and ICR strains were cryopreserved using 18 % raffinose, 3 % skim milk as cryoprotectant aolution, and estimated the survival and fertility of post-thawed sperm from the five strains. The progressive motility of sperms from each strain was affected differently. Results The fertility rate of C57BL/6J, FVB/NJ, B6D2F1, KM and ICR strains were significantly decreased from 81.4%, 87.2%, 90.4%, 82.7% and 79.4% , respectively, of fresh sperm, to 6.7%, 46.0%, 76.8% , 59.2% and 31.9%, respectively, when using post-thawed sperms（ P 〈 0.01 ）. Conclusion The fertility and susceptibility to eryopreservation of sperm from mice of different genetic background differ significantly.

关 键 词：受精 体外 小鼠 冻融精子 

分 类 号：R-33[医药卫生]