胃癌组织中p53基因突变和p53蛋白表达  

The Mutation of p53 Gene and Expression of p53 Protein in Gastric Cancer

作　　者：贺岩[1] 张磊[1] 叶菲[1] 金晓明[1] 

机构地区：[1]哈尔滨医科大学病理教研室,哈尔滨150086

出　　处：《中国比较医学杂志》2006年第12期740-743,共4页Chinese Journal of Comparative Medicine

基　　金：黑龙江省青年科学技术专项资金(QC05C38);黑龙江省教育厅资金(10541105)

摘　　要：目的比较胃癌和癌旁组织中p53基因突变及p53蛋白的表达与淋巴结转移和预后的关系。方法采用PCR-SSCP技术和免疫组织化学S-P法,对石蜡包埋组织进行p53基因外显子6、7和8,9以及p53蛋白的检测。结果(1)p53蛋白的阳性表达在癌组织和癌旁组织中分别为47.6%和38.1%;有淋巴结转移的病例p53蛋白表达52.8%。(2)癌组织中p53外显子7的突变率为23.9%;癌旁组织中突变率为19.0%。癌组织中p53外显子6的突变率为10.5%;癌旁组织为8.6%。癌组织中p53外显子8,9的突变率为1.9%;癌旁组织为1.0%。结论有淋巴结转移的病例中,p53外显子的突变率和蛋白的表达率均明显增高,提示p53外显子的突变在胃癌的发生发展中是一个重要因素,并促进肿瘤的转移。p53基因突变与p53蛋白高表达在癌组织和癌旁组织中无显著差异。Objective To study the relationship between lymph node metastasis and prognosis and the p53 gene mutation and expression of p53 protein in carcinoma and paracancerous tissues of the stomach. Methods The mutation of p53 gene and expression of p53 protein were examined by PCR-SSCP and immunohistochemical staining techniques. Results （1） The positive expression rate of p.53 protein was 47.6% and 38.1% in carcinomas and paracanerous tissues, respectively. The expression of p53 protein reached to 52.8% . （2） The mutation rate of the exon 7 was 23.9% and 19.0% in paraffin-embedded carcinomas and paracancerous tissues, respectively. The mutation rate of exon 6 was 10.5 % and 8.6% in paraffin-embedded carcinomas and paracancerous tissues, respectively. The mutation rate of exon 8, 9 was 1.9% and 1.0% in paraffin-embedded carcinomas and paracancerous tissues, respectively. Conclusion In cases with lymph node metastasis, the mutation of p53 gene and expression of p53 protein was apparently increased, indicating that the mutation of exons in the development and progression of gastric carcinoma is an important factor. It also facilitates the metastasis of tumor. The mutation of p53 gene and high expression of p53 protein are not significantly different in carcinomas and paracancerous tissues of the stomach.

关 键 词：胃癌 癌旁组织 p53外显子 P53蛋白 PCR-SSCP 

分 类 号：R-33[医药卫生]