棕环海参化学成分的研究  

Studies on chemical constituents of the sea cucumber Holothuria fuscocinerea

作　　者：张淑瑜[1] 汤海峰[2] 易杨华[1] 刘世君[3] 张卫星[3] 胡冠时[3] 

机构地区：[1]第二军医大学药学院海洋药物研究中心,上海200433 [2]第四军医大学西京医院药剂科,陕西西安710032 [3]济南军区总医院药剂科,山东济南250031

出　　处：《中国海洋药物》2006年第6期7-13,共7页Chinese Journal of Marine Drugs

摘　　要：目的研究中国南海棕环海参的化学成分。方法采用稻瘟霉模型生物活性追踪方法,应用多种色谱技术分离,根据波谱解析和化学手段鉴定化合物的结构。结果从棕环海参正丁醇萃取物中分离鉴定了3个三萜皂苷:pervicoside C(Ⅰ),holothurin A(Ⅱ)和DS-holoturin B(Ⅲ);从氯仿萃取物中分离鉴定了4个化合物:尿嘧啶(Ⅳ),胸腺嘧啶(Ⅴ),胸腺嘧啶脱氧核苷(Ⅵ)和尿嘧啶脱氧核苷(Ⅶ)。结论所有化合物均为首次从该种海参中获得,化合物I为新天然产物,Ⅰ和Ⅱ具有诱导稻瘟霉菌丝变形活性和肿瘤细胞毒性。Objective To study the chemical constituents of the sea cucumber Holothuria fuscocinerea collected from the South China Sea. Methods The compounds were isolated by Pyricularia oryzae bioassay-guided fractionation method in combination with extraction and partition as well as multi-chromatography. Their structures were determined on the hasis of spectral analysis and chemical evidence. Results Three triterpene glycosides were isolated from the n-BuOH extract and identified as： pervicoside C （Ⅰ）, holothurin A （Ⅱ） and DS-holoturin B （Ⅲ）, Four compounds [uracil （Ⅳ）, thymine （Ⅴ）, thymidine （Ⅵ） and 2-deoxyuridine （Ⅶ）] were obtained from the CHCla extract. Conclusion All the compounds were isolated from this sea cucumber for the first time while Compound Ⅰ was first obtained as pure compound. Compound Ⅰ and Ⅱ were active compounds causing morphological abnormality of P. oryzae mycelia and exhibited cytotoxicity against two cancer cell lines .

关 键 词：海参 棕环海参 三萜皂苷 稻瘟霉 细胞毒性 

分 类 号：Q959.26[生物学—动物学]