微波辅助碱解荧光分光光度法快速测定河豚毒素含量  

Rapid Determination of Tetrodotoxin Using Microwave Assisted Alkaline Hydrolysis and Spectrofluorometric Analysis

作　　者：沈晓书[1] 郭磊[1] 顾明松[1] 冯建林[1] 谢剑炜[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《光谱学与光谱分析》2006年第12期2264-2267,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20305013)资助

摘　　要：建立了一种快速、简便、灵敏地测定河豚毒素的新方法。在水和异丙醇混合碱性溶液中对河豚毒素进行微波碱解，通过测定碱解产物的量来间接测定河豚毒素的含量。结果表明：C9碱的最大激发波长为380m，最大发射波长为496m，其在0．1～10μmol·L^-1的浓度范围内与荧光强度呈线性关系，相关系数r=0．9991，检测限为0．05μmol·L^-1比文献报道降低近20倍。该方法快速、灵敏和准确，可用于河豚毒素的定量检测。To develop a rapid, simple and sensitive method for determining the concentration of tetrodotoxin（TTX）, TTX was hydrolyzed in the strong alkali solution of water mixed with isopropanol. The concentration of TTX can be indirectly analyzed by the fluorescence signal of its alkaline hydrolysis product which can be enhanced by the microwave method. The maximum excitation and emission wavelengths of the alkaline hydrolysis product of tetrodotoxin were 380 and 496 nm, respectively. The linear range of the calibration curve was 0. 1-10μmol·L^-1 with r=0. 9991. The limit of detection was 0. 05 μmol·L^-1 , which was twenty times lower than before. A rapid, highly sensitive and accurate method was thus established. It can be used as a quantitative method for detecting TTX.

关 键 词：河豚毒素 荧光分光光度法 微波碱解 

分 类 号：R996[医药卫生—毒理学]