DMBA诱导的金黄地鼠颊囊癌变粘膜中增殖细胞核抗原表达  

A study on change of proliferating cell nuclear(PCNA) during DMBA induce golden hamster cheek pouch carcinoma

作　　者：周永梅[1] 周曾同[1] 王英[1] 曹宏康[1] 

机构地区：[1]上海第二医科大学口腔医学院口腔内科教研室

出　　处：《上海口腔医学》1996年第4期207-209,共3页Shanghai Journal of Stomatology

摘　　要：实验采用抗增殖细胞核抗原单克隆抗体对１０９例金黄地鼠颊囊粘膜标本作免疫组化染色及网格计数和形态学观察，发现正常的金黄地鼠颊囊粘膜基底层有少量增殖细胞核抗原（ＰＣＮＡ）阳性细胞，而上皮异常增生以及癌变时，随着病变程度的加重，ＰＣＮＡ阳性细胞率也相应增加。统计学分析显示：正常粘膜各级上皮异常增生以及癌变各组间ＰＣＮＡ阳性细胞率有显著差异（Ｐ＜０．０５），轻度上皮异常增生组中涂ＤＭＢＡ６周与７周的ＰＣＮＡ阳性分级有显著性差异（Ｐ＜０．０５）；涂ＤＭＢＡ７周，８周，９周之间，其ＰＣＮＡ阳性分级无差异（Ｐ＞０．０５），提示ＰＣＮＡ可作为动态观察癌前病变过程中细胞增殖程度的一种检测指标，它的敏感性强于光镜形态学观察。Abstract cases were studied by immunohistochemisty with anti-PCNA antibody specimens wihch dyed by PCNA immunohistochemistry were counted with net and morphology observed.The results indicated that:a few of PCNA-positive cells had been seen in the basal cells of normal epithlium. An increased percentage of PCNA-positive cells was found in dysplasia and carcinoma. The increase between every grade is of statistical:significance(p<0.05).In slight dysplasia grade group,the percentagc of PCNA-positive cells increasing between applying DMBA-six-weeks' group is of statistcal significance(p<0.05),and hadn't found statistical difference among the other groups. It is demonstrated that PCNA is one of criteria which were observed cell reproduction reactivities during precarcinoma,and it is more sensitive than light microscopy.

关 键 词：金黄地鼠 颊囊 增殖细胞核抗原 癌变粘膜 

分 类 号：R739.8[医药卫生—肿瘤]