泥浆体系中吡啶的生物降解研究  

Biodegradation of pyridine in the slurry system

作　　者：刘江江[1] 陈吕军[1] 温东辉[2] 何玉山[2] 乔琳 夏勉 李毅[4] 

机构地区：[1]清华大学环境科学与工程系//环境模拟与污染控制国家联合重点实验室,北京100084 [2]北京大学环境学院环境科学系,北京100871 [3]北京未名凯拓农业生物技术有限公司,北京100089 [4]北京大学生命科学学院生物技术系,北京100871

出　　处：《生态环境》2006年第6期1180-1184,共5页Ecology and Environmnet

基　　金：国家科技部"863"前沿探索类课题(2004AA649090)

摘　　要：杂环化合物已造成了土壤、地下水等体系的严重污染,危害人类健康与生态环境。生物修复是解决该类污染问题的有效方法。由实验室培养的活性污泥中分离一株高效降解吡啶的菌株W12,经鉴定为脱氮副球菌(Paracoccusdenitrificans),以W12菌对受吡啶污染的泥浆体系进行生物修复。实验条件下,土壤对吡啶的吸附量很小,符合Freundlich吸附等温式。当吡啶初始质量分数为1.65mg·g-1时,投加W12菌能够迅速促进吡啶的生物降解,在灭菌土和自然土中完全降解吡啶的时间分别为12h和18h,说明W12菌在自然土中的降解效果受到土著微生物的竞争影响;此外,对泥浆体系中吡啶降解的影响因素进行了研究,发现投菌量是影响吡啶降解速率的关键因素,外加氮源以及土水比均对吡啶降解过程影响不大。The pollution of soil and underground water caused by nitrogen heterocyclic aromatics are increasingly serious nowadays. Bioremediation seems a promising solution to this problem. A bacteria strain W12, which could utilize pyridine as the sole carbon source and nitrogen source, was isolated from the active sludge in a lab bioreactor, and identified as species of Paracoccus denitrificans. In this study, we applied this strain in the slurry bioremediation in which the soil was simulated as polluted by pyridine, Under the experimental conditions, the adsorption of pyridine onto the soil was very weak, but fitting the Freundlich isotherm. When the initial concentration ofpyridine was 1.65 mg·g^-1, the inoculated strain could degrade the pyridine thoroughly within 12 h and 18 h in the sterilized soil and natural soil, respectivdy. It showed that the pyridine degradation efficiency by the strain W12 was depressed by the soil indigenous microbes because of food limitation. In addition, the influence of the delivering amount of the strain W12 as well as the external nitrogen resources and the soil-water-ratio of the slurry were studied. It showed that the delivering amount was an important factor to influence the biodegradation of pyridine in the slurry, while the external nitrogen resources and the soil-water-ratio had little effects on the biodegradation.

关 键 词：脱氮副球菌 生物降解 吡啶 生物修复 泥浆 

分 类 号：X172[环境科学与工程—环境科学]