5-氟尿嘧啶诱发人支气管上皮损伤前后P糖蛋白表达的研究  

Expression of P-glycoprotein before and after human bronchial epithelium injury induced by 5-fluorouracil

作　　者：周莹[1] 贾心善[2] 廖爱军[3] 

机构地区：[1]中国医科大学附属第一医院急诊科,辽宁沈阳110001 [2]中国医科大学基础医学院病理学教研室,辽宁沈阳110001 [3]中国医科大学附属第二医院血液科,辽宁沈阳110001

出　　处：《中国医科大学学报》2006年第1期28-30,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30170407)

摘　　要：目的:研究离体人支气管上皮经5-氟尿嘧啶(5-FU)损伤前后P糖蛋白(P-gp)的表达。方法:5-FU造成离体人支气管环严重损伤,分别取正常组及5-FU作用后的支气管上皮,应用流式细胞仪和蛋白印迹法检测P-gp的表达;RT-PCR法检测其mRNA的表达。结果:5-FU作用后支气管上皮细胞中P-gp阳性细胞所占比例较正常组增高,在蛋白及mRNA水平,5-FU作用后支气管上皮P-gp的表达均较正常组增加。结论:5-FU作用后,表达P-gp的支气管干细胞占残留活细胞的比例增多,由它们完成损伤的修复。Objective： To explore the expression of P-glycopmtein （P-gp） before and after the isolated human bronchial epithelium injury induced by 5-fluomuraeil （5-FU）. Methods： The isolated human bronchial epithelium was injured by 5-FU. The expression of P-gp in 5-FU group and control group was detected by flow cytometry and Western Blotting. The expression of P-gp mRNA was detected by reverse transefiptase polymerase chain reaction （RT-PCR）. Results： The proportion of P-gp positive cells and the expression of P- gp in 5-FU group were higher than those in control group. Conduslons： The proportion of bronchial stem cells with P-gp expression increases after administration of 5-FU. These bronchial stem cells participate in the repair of bronchial epithelial injury.

关 键 词：支气管 干细胞 5-氟尿嘧啶 人 P糖蛋白 

分 类 号：R36[医药卫生—病理学]