P_(53)蛋白对人瘢痕疙瘩成纤维细胞端粒酶活性的影响  

Experimental research on the effect of P_(53) protein on telomerase activity in human keloid fibroblasts

作　　者：毕国华[1] 张淼波[2] 肖志波[2] 

机构地区：[1]哈尔滨医科大学附属第四医院整形外科,哈尔滨150001 [2]哈尔滨医科大学附属第二医院整形外科,150001

出　　处：《中国烧伤创疡杂志》2006年第4期252-255,共4页The Chinese Journal of Burns Wounds & Surface Ulcers

基　　金：哈尔滨医科大学博士研究课题

摘　　要：目的:探讨P53蛋白(P53 protein)对人瘢痕疙瘩成纤维细胞(Keloid fibroblast／KFB)端粒酶活性的影响;明确在人瘢痕疙瘩成纤维细胞中P53蛋白与端粒酶活性之间的相互关系。方法:采用腺病毒介导法将野生型P53基因(Ad-P53)转染至人瘢痕疙瘩成纤维细胞中;将来源于瘢痕疙瘩组织的成纤维细胞随机分成二组。实验组转染野生型P53基因至成纤维细胞中。非转染组的成纤维细胞未进行野生型P53基因转染。用间接免疫荧光法检测成纤维细胞中P53蛋白的表达;TRAP-ELISA法检测成纤维细胞中端粒酶活性。结果:转染组细胞中P53蛋白表达明显高于非转染组细胞;而端粒酶活性明显低于非转染组细胞(P<0．01)。结论:P53蛋白能够抑制人瘢痕疙瘩成纤维细胞端粒酶活性。Objective： To investigate the effect of P53 protein on telomerase activity in human keloid fibroblasts. Method： Fibroblasts derived from keloid tissue were transfected by adenovirus - wt - P53 gene. The expression of P53 protein was checked by immunofluorescence. The activity of telomerase was checked by TRAP - ELISA and compared with that of fibroblasts not transfected by adenovirus - wt - P53 gene. Result： In fibroblasts transfected by adenovirus - wt - P53 gene, the expression of P53 protein was higher and the telomerase activity was lower than that in not transfected fibroblasts （P 〈0. 01 ）. Conclusion： P53 protein significantly inhibits telomerase activity in human keloid fibroblasts.

关 键 词：P53 端粒酶 转染 瘢痕 

分 类 号：R739.5[医药卫生—肿瘤]