基于Survivin的模拟病毒瘤苗体外激发CTL杀伤活性的实验研究  

Cytotoxic activity of CTL stimulated by mimovirus tumor vaccine based on Survivin in vitro

作　　者：杨空明 牛微[1] 李晋涛[1] 吴玉章[1] 赵建平[1] 王莉[1] 

机构地区：[1]第三军医大学基础部全军免疫研究所,重庆400038

出　　处：《中华肿瘤防治杂志》2006年第23期1761-1764,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30400437;30400402)

摘　　要：目的探讨基于Survivin的模拟病毒瘤苗体外激发细胞毒性T细胞(cytotoxicTlymphocytes,CTL)对乳腺癌细胞的杀伤作用。方法制备含有Survivin的模拟病毒瘤苗,体外刺激HLA-A2·1阳性健康者外周血单个核细胞(peripheralbloodmononu-clearcell,PBMC),用酶联免疫斑点实验(enzymelinkedimmunospotassay,ELISPOT)和标准51Cr释放实验分别检测瘤苗所诱导的特异性CTL活性。结果Survivin模拟病毒瘤苗能够在体外诱导出特异性CTL,对HLA-A2·1阳性的乳腺癌细胞系MCF-7有明显的杀伤毒性,杀伤率为56·4%;并可诱导CTL产生IFN-γ等细胞因子,ELISPOT显示斑点数为287个/105细胞,P<0·05。结论模拟病毒瘤苗能有效激发出特异性CTL应答,为新型乳腺癌治疗性疫苗的设计提供了新思路。OBJECTIVE： To discuss the cytotoxie activity of CTL, stimulated by mimovirus tumor vaccine based on Survivin in vitro, to breast caner ceils. METHODS： Mimovirus tumor vaccine based on Survivin was prepared, and PBMC of HLA-A2.1 positive blood donors was induced in vitro. The specific cytotoxic activity of CTL was measured by ELISPOT and standard 4 h ^51Cr release assay. RESULTS： The tumor vaccine could induce specific CTL which showed specific cytotoxic effect to HLA-A2.1 positive breast cancer cells MCF-7 （KP=56.4%） and produced IFN-γ with ELISPOT number of 287 per 10s cells, P〈0.05. CONCLUSIONS： The tumor vaccine can induce specific CTL response. This result maybe can provide a new therapeutic vaccine for breast caner patient.

关 键 词：癌症疫苗 T淋巴细胞 细胞毒性 乳腺肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]