9-顺维甲酸诱导肺癌组织RARβ转录的研究  

Study on 9-cic-retinic acid-induced RARβ transcription in lung tumor tissue

作　　者：戴洪海[1] 陈萍[2] 胡国强[3] 于甬华[1] 于金明[1] 

机构地区：[1]山东省肿瘤医院特需二科,山东济南250117 [2]济南市中心医院血液科,山东济南250013 [3]山东大学齐鲁医院胸外科,山东济南250012

出　　处：《中华肿瘤防治杂志》2006年第21期1605-1608,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(39770730)

摘　　要：目的:观察9-顺维甲酸(9-cic-retinicacid,9-cis-RA)对肺癌组织和对照肺组织RARβ转录水平的影响。方法:应用组织块法培养肺癌组织和对照肺组织,并同时应用冷消化法原代培养肺癌细胞,观察培养组织中细胞的活力,采用RT-PCR法检测9-cis-RA处理24h前后RARβ的mRNA表达水平。结果:9-cis-RA处理前非小细胞肺癌(NSCLC)组和小细胞肺癌(SCLC)组肺癌组织RARβ表达水平(0.5216±0.1671,0.5829±0.2116)较其对照肺组织(0.7236±0.1261,0.8432±0.0621)下降,P<0.05。经9-cis-RA处理24h后,NSCLC和SCLC组对照肺组织RARβ转录水平(0.7789±0.1326,0.8161±0.5102)较加药前(0.7236±0.1261,0.8432±0.0621)变化差异无统计学意义,P>0.05。肺癌组织的RARβ的转录水平(0.7691±0.1366,0.7792±0.1766)较加药前(0.5216±0.1671,0.5829±0.2116)显著升高,P<0.05。并且升高后的转录水平与对照肺组织的基础转录水平相近,NSCLC组(0.7691±0.1366)和SCLC组(0.7792±0.1766)间肺癌组织的RARβ的转录水平差异无统计学意义,P>0.05。结论:RARβ的表达下降可能与肺癌的发生发展有关。9-cis-RA可诱导肺癌组织中RARβ的表达水平升高并且达到对照肺组织的表达水平。OBJECTIVE：To observe the effects of 9-cisretinoic acid on RARβ in lung cancer tissue. METHODS： Tumor tissue and control lung tissue were cultured by the tissue mass method while lung cancer cells were cultured by the cold digestion method to observe the viability of cell in the tissue. RARβ mRNA levels of the tissue treated with 9-cis-RA for 24 h were detected by RT-PCR method. RESULTS： RARβ transcription levels were lower in lung cancer tissue of NSCLC and SCLC （0. 521 6±0. 167 1, 0. 582 9±0. 211 6） than those in normal controls （0. 723 6±0. 126 1, 0.843 2±0.062 1）, P〈0. 05. After being treated with 9-cis-RA for 24 h, transcriptional levels of RARβ in the normal controls （0. 778 9 ±0. 132 6,0. 816 1±0. 510 2） showed no difference from levels before the treatment （0. 723 6±0. 126 1, 0. 843 2±0. 062 1）, P〉0. 05. RARβ transcriptional levels increased significantly in the tumor tissues of NSCLC and SCLC（0. 778 9±0. 132 6, 0. 816 1±0. 510 2） compared with the levels before the treatment （0.521 6±0.167 1, 0.582 9±0.211 6）, P〈0.05, reaching the transcriptional levels in the normal controls （0.723 6±0.126 1, 0.843 2±0.062 1）. There were no difference between the transcriptional levels of RARβ in NSCLC （0. 769 1±0. 136 6） and SCLC（0. 779 2±0. 176 6）, P〉 0. 05. CONCLUSION： Lower expression of RARβ in lung cancer tissue may be associated with lung carcinogenesis and development; 9-cis-RA can induce expression of RARβ in lung cancer tissue to the transcriptional level in controll lung tissue.

关 键 词：肺肿瘤/病理学 抗肿瘤药/药理学 维甲酸/药理学 肿瘤细胞 培养的 

分 类 号：R734.2[医药卫生—肿瘤]