ICAM基因多态性与BRCA1相互作用对乳腺癌危险度的影响  

Effect of interaction between polymorphisms in the ICAM gene locus and BRCA1 on breast cancer risk

作　　者：蒋东华[1] WILLIAM W Zhang 李霓[1] 周宝森[1] 

机构地区：[1]中国医科大学公共卫生学院流行病学教研室,辽宁沈阳110001 [2]Center for Breast Cancer Research on Women's Health,Sunnybrook & Women's College Health Science Center,Toronto,M5G 1N8,Canada

出　　处：《中华肿瘤防治杂志》2006年第21期1609-1613,共5页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨细胞间黏附分子(ICAM)基因座位单核苷酸多态性(SNPs)与乳腺癌危险度之间的关系,以及这些SNPs位点与BRCA1基因相互作用对乳腺癌危险度的影响。方法:研究对象为1034例女性乳腺癌病例和1091例非恶性肿瘤对照。采外周血提DNA后使用基质辅助激光解吸附电离飞行时间质谱检测技术(MALDI-TOF)对SNPs位点进行基因分型。用χ2检验和Logistic回归模型进行统计学分析。结果:rs1056538位点携带C等位基因对个体患乳腺癌具有保护作用(ORCT=0.3423,95%CI:0.2351～0.4982;ORCC=0.5943,95%CI:0.3899～0.9058),而rs2228615AG和rs281439GG基因型个体则有较高的危险度发生乳腺癌(ORrs2228615AG=2.6771,95%CI:1.4938～4.7976;ORrs281439GG=1.9845,95%CI:1.0287～3.8285)。对于携带BRCA1突变的个体,与全部研究对象所得的结果相比,rs1056538位点携带C等位基因的保护作用更为明显(ORCT=0.2797,95%CI:0.1984～0.3321;ORCC=0.4983,95%CI:0.3942～0.7211),rs2228615位点携带G等位基因的危险度更高(ORAG=3.3369,95%CI:1.9195～5.8009;ORGG=2.0289,95%CI:1.1704～3.5170),而rs281439GG基因型危险度改变不大。结论:ICAM基因座位上SNPs与乳腺癌危险度有关。ICAM基因与BRCA1基因间存在相互作用,影响了乳腺癌的危险度。OBJECTIVE：To explore the associations between polymorphisms in ICAM gene locus and breast cancer risk,and the effect of interactions between SNPs and BRCA1 on breast cancer risk. METHODS： There were 1 034 breast cancer cases and 1 091 controls in this study. After DNA was isolated from peripheral blood, the matrix-assisted laser de sorption/ionization time-of-flight mass spectrometry （MALDI-TOF）was used to determine the genotype of every SNPs. χ^2 test and logistic regression model were used to.analyze the association. RESULTS： Rs1056538 with C allele might have some prevention effect on women breast cancer risk （ORCT=0.342 3, 95%CI： 0.235 1-0.498 2; ORCC= 0. 594 3, 95%CI： 0. 389 9-0. 905 8）, while women whose genotype was rs2228615AG or rs281439GG would have higher breast cancer risk（OR rs2228615AG = 2. 677 1, 95% CI： 1.493 8-4.797 6; OR rs281439GG= 1.984 5, 95%CI： 1. 028 7-3. 828 5）. For women with BRCA1 mutation, compared with the results of all subjects, rs1056538 with C allele might have greater prevention effect （ORCT = 0. 279 7, 95%CI： 0.198 4- 0.332 1; ORCC = 0.498 3, 95% CI： 0. 394 2-0. 721 1）, while rs222861 with G allele would have higher breast cancer risk （ORAG = 3.336 9, 95% CI： 1.919 5-5.800 9; ORGG=2.028 9, 95%CI：1.170 4- 3. 517 0）, but rs281439GG genotype had little diffenence in the effect on the risk. CONCLUSION： There is an association between ICAM gene locus SNPs and breast cancer risk. The interaction between ICAM gene and BRCA1 has some effect on breast cancer risk.

关 键 词：乳腺肿瘤 细胞黏附分子 BRCA1 多态性 

分 类 号：R737.9[医药卫生—肿瘤]