咬合重建对大鼠颞下颌关节降钙素基因相关肽表达的影响  

Expressions of calcitonin gene-related peptide in rats with occlusal reconstruction

作　　者：李晓光[1] 袁丽丽[1] 刘慧娟[2] 王延秀[3] 

机构地区：[1]泰安市中心医院口腔科,山东泰安271000 [2]泰安市中心医院手术室,山东泰安271000 [3]泰安市中心医院麻醉科,山东泰安271000

出　　处：《上海口腔医学》2006年第6期649-652,共4页Shanghai Journal of Stomatology

基　　金：泰安市科技局资助项目(2003-52)

摘　　要：目的研究咬合重建对大鼠颞下颌关节降钙素基因相关肽(calcitoningene-relatedpeptide,CGRP)表达的影响。方法Wistar雄性大鼠30只,随机分为3个实验组及相应的对照组,每组5只。实验组动物间断磨除右上、下颌磨牙牙冠至龈下,其中2组分别于第3周、第9周停止磨牙,任其自行萌出,恢复咬合关系。颞下颌关节切片行CGRP免疫组织化学反应(SABC法)。光镜观察拍片,用ImageProPlus5.1图像分析软件进行测定。结果与对照组比较,以SPSS10.0统计软件包进行分析。结果单侧咀嚼实验组,咀嚼侧和非咀嚼侧颞下颌关节单位面积内CGRP阳性纤维面积与对照组比较显著增加(P<0.01,P<0.05),其非咀嚼侧单位面积内CGRP阳性纤维面积显著高于咀嚼侧(P<0.05)。早期恢复咬合实验组,CGRP阳性纤维面积与对照组比较无显著差别(P>0.05),其咀嚼侧与非咀嚼侧比较亦无显著差别(P>0.05)。晚期恢复咬合实验组CGRP阳性纤维面积与对照组比较显著增加(P<0.01),其非咀嚼侧单位面积内CGRP阳性纤维面积显著高于咀嚼侧(P<0.05)。结论早期恢复咬合关系后,颞下颌关节内CGRP表达可恢复正常;晚期恢复咬合关系,CGRP表达不能恢复正常,CGRP参与了单侧咀嚼引起的颞下颌关节病的病理变化过程。PURPOSE： To investigate the expressions of calcitonin gene-related peptide（CGRP） in rats with occlusal reconstruction. METHODS： 30 Wistar male rats were randomly divided into 3 experimental groups and 3 control groups, 5 rats in each group.The molar of the right maxilla and mandible of rats in the experimental groups were ground to the gingival level without occlusal contact. The occlusal contact was recovered by stoping grounding molar of the rats in two of the experimental groups.The section of the disc and articular capsule was subject to immunohistological study to evaluate the expressions of CGRP by using SABC method.Light microscope and microscopic photo analytic software were employed to detect calcitonin gene-related peptide-like immunoreactive（CGRP-LI） nerve fibers in frozen section of TMJs in 30 rats. SPSS10.0 software package were used for statistical analysis. RESULTS： The areas of CGRP-LI nerve fibers in unilateral chew group were significantly larger than those in the control group （P〈0.01,P〈0.05）.The increment in the non-chewing side was significantly higher than that in the chewing side（P〈0.05）.There was no significant difference in the areas of CGRP-LI nerve fibers between the early oeclusal reconstruction group and the control group （P〉0.05） and there was no significant difference between the non-chewing side and the chewing side（P〉0.05）. There was significant difference in the areas of CGRP-LI nerve fibers between the later occlusal reconstruction group and the control group（P〈0.01）,while no significant difference was found between the non-chewing side and the chewing side（P〉0.05）. CONCLUSION： The expressions of CGRP can recover normal in early occlusal reconstruction but not in later oeclusal reconstruction.CGRP might participate in the histopathogenesis of temporomandibular joint disorders. Supported by Research Fund from Science and Technology Bureau of Taian City （Grant No.2003-52）.

关 键 词：降钙素基因相关肽 颞下颌关节 咬合重建 单侧咀嚼 大鼠 

分 类 号：R782.6[医药卫生—口腔医学]