雷公藤内酯醇对血管内皮细胞t-PA、PAI-1蛋白表达的影响  

Effects of triptolide on t-PA and PAI-1 protein expression in vascular endothelial cells

作　　者：曹永亮[1] 张杰[1] 赵庆亮 丁怡[3] 王杰[1] 赵岩松[1] 

机构地区：[1]潍坊医学院附属医院眼科中心,山东省潍坊市261031 [2]鲁南眼科医院,山东省临沂市276000 [3]潍坊医学院病理生理教研室,山东省潍坊市261031

出　　处：《眼科新进展》2007年第1期15-18,共4页Recent Advances in Ophthalmology

基　　金：山东省教育厅重点资助课题(编号:J01K08)~~

摘　　要：目的研究雷公藤内酯醇(triptolid,T10)对血管内皮细胞移行活性及组织型纤溶酶原激活物(tissue-typeplasminogenactivator,t-PA)和纤溶酶原激活物抑制物-1(plas-minogenactivatorinhibitor-1,PAI-1)蛋白表达的影响,探讨T10对新生血管生成的抑制作用。方法体外培养传3代人脐静脉内皮细胞(humanumbilicalveinendothelialcells,HUVECs),加入不同浓度的T10(5μg·L-1、10μg·L-1、20μg·L-1、30μg·L-1)、地塞米松(300mg·L-1),观察HUVECs移行活性;免疫组织化学染色检测HUVECs中t-PA和PAI-1蛋白表达量。结果T10能明显抑制HUVECs移行活性,使HUVECs移行数量、移行距离明显减少和缩短;T10能明显抑制HUVECs中t-PA和PAI-1蛋白表达,使t-PA和PAI-1蛋白表达棕黄色阳性染色减少,与对照组相比差异具有显著性(P<0.01)。结论T10能明显抑制血管内皮细胞移行,抑制内皮细胞t-PA和PAI-1蛋白表达,从而有效抑制新生血管生成与生长。Objective To observe the effects of trtptolide on the migration activity,protein expression of tissue-type plasminogen activator（t-PA） and. plasminogen activator inhibitor-1 （ PAI-1 ） of human umbilical vein endothelial cells （HUVECs） cultured in vitro, and elucidate the anti-angiogenic mechanism of triptolide. Methods HUVECs were cultured in vitro and the third passage cells were treated with triptolide （5μg · L^-1,10μg · L^-1,20μg · L^-1,30μg · L^-1） ,or dexamethasone （ 300 mg·L^-1 ） as control. The effects of triptolide on the migration of HUVECs were observed,and immunohistochemical assay was applied to detect protein expression of t-PA and PAI-1. Results Triptolide inhibited the migration activity obviously, shortened the migrated distance and reduced the number of HUVECs. Triptolide suppresed the protein expression of t-PA and PAI-1 ,and reduced the brown positive staining. There were obvious differences compared with the control group（P 〈0. 01 ）. Conclusion Triptolide can inhibit the migration activity of HUVECs,and down-regulate protein expression of t-PA and PAI-1 ,so it can inhibit the angiogenesis effectively.

关 键 词：雷公藤内酯醇 血管内皮细胞 组织型纤溶酶原激活物 纤溶酶原激活物抑制物-1 蛋白表达 血管生成 

分 类 号：R77[医药卫生—眼科] R364[医药卫生—临床医学]