新型PCR-SSO方法在分析Eales病患者HLA-B基因型中的应用  

Application of new PCR-SSO method in analysis of HLA-B genetype in Eales' disease

作　　者：赵军[1] 魏世辉[2] 胡莲娜[1] 

机构地区：[1]解放军306医院眼科,北京市100101 [2]解放军301医院眼科,北京市100853

出　　处：《眼科新进展》2007年第1期41-43,共3页Recent Advances in Ophthalmology

基　　金：全军"十五"科研基金资助(编号:04M042);吴阶平医学基金会资助(编号:2003-32-D)~~

摘　　要：目的通过使用新型聚合酶链反应-序列特异的寡核苷酸扩增分析(polymerasechainreactionrestrictionsequencespecificoligonucleotide,PCR-SSO)方法分析Eales病患者及正常人群的人类白细胞抗原B组(humanleucocyteantigen-B,HLA-B)基因型,了解这种方法的特点及其优越性。方法选取25例已确诊Eales病的患者作为研究组,自献血血库中选取与研究组患者年龄、性别无统计学差异的25例健康人作为对照组。抽取外周血提取DNA。使用新型PCR-SSO方法对DNA进行HLA-B基因位点的检测,得出结果,并进行统计学分析。结果分析结果可以同时显示基因型及对应的血清型,发现对照组中基因型HLA-B*15基因位点及对应的HLA-B62血清型明显高于研究组(P<0.01)。结论PCR-SSO方法是一种先进的基因分型技术,具有其他基因分型方法无法比拟的优势,在科研工作中具有良好的应用前景。Objective To understand the characteristic and merit of new polymerase chain reaction restriction sequence specific oligonacleotide （PCR-SSO） technique by analyzing the human leucocyte antigen-B（HLA-B） genetype in Eales＇ disease and control group. Methods Twenty-five Eales＇ disease patients were selected as research group ,and 25 healthy people from blood-devote center were selected as control group. DNA was gained from their peripheral blood,and HLA-B allele was determined by new PCR-SSO method. The results were studied statistically. Results Genetype and corresponding serotype gave out at the same time, and genetype HLA-B^＊ 15 and HLA-B62 serotype in control group was significant（P 〈 0. 01 ） higher than that in research group. Conclusion New PCR-SSO method is better than other ordinary methods and has good prospect in the future.

关 键 词：序列特异寡核苷酸扩增分析 EALES病 人类白细胞抗原 

分 类 号：R774[医药卫生—眼科]