阿霉素诱导SACC-83细胞凋亡的试验研究  

The study of apoptosis induced by different dose doxorubicin hydrochloride in SACC-83 cell

作　　者：李旭奎[1] 周昌龙[2] 饶国州[1] 张引成[1] 

机构地区：[1]西安交通大学口腔医学院,陕西西安710004 [2]浙江省宁波市医疗中心李惠利医院口腔科,浙江宁波315000

出　　处：《现代肿瘤医学》2007年第1期13-15,共3页Journal of Modern Oncology

摘　　要：目的:探讨阿霉素诱导腺样囊性癌细胞凋亡的最佳剂量。方法:采用SACC-83细胞系,于体外分别以含有5μg/ml、10μg/ml、20μg/ml阿霉素的培养液处理12小时后,进行凋亡DNA电泳梯状图谱、流式细胞仪凋亡率的检测和细胞形态学分析。结果:5μg/ml、10μg/ml组细胞检测到凋亡带,而20μg/ml组可见明显的坏死带。形态学上看到20μg/ml组主要表现为坏死;10μg/ml凋亡与坏死同时存在,;5μg/ml时表现为单纯的细胞凋亡。流式细胞仪检测结果发现,在5μg/ml浓度阿霉素作用下,凋亡细胞可达68.46%与其它组相比有显著性差异(P<0.01)。结论:通过阿霉素诱导SACC-83细胞建立凋亡模型,检测发现阿霉素5μg/ml作用下可得到较高含量的凋亡细胞。Objective： To study the most favorable dose of doxorubicin that induced apoptosis of salivary adenoid cystic carcinoma line cells（ SASS - 83 ）. Methods： Salivary adenoid cystic carcinoma line cells（ SASS - 83） were cultured with different concentration of doxorubicin. Apoptosis were studied by DNA electrophoresis, morphological characteristics, quantitatively analyzed with FCM after cultured with doxorubicin at the final 5μg /ml, 10μg/ml,20μg/ml concentration for 12 hours . Results： The doxorubicin induced necrosis of SACC - 83 cell line increased with concentration ranging from 5μg/ml to 20μg/ml . The final concentration 5 μg/ml doxorubicin that was significantly differet from the other groups （P 〈 0.01 ）. Conclusion： The concentration of doxorubicin in 5 μg/ml is the best that induces apoptosis of SASS - 83 cells.

关 键 词：阿霉素 凋亡 腺样囊性癌 

分 类 号：R730[医药卫生—肿瘤]