硒化紫球藻胞外多糖组成与结构的初步分析  

The Primary Research of the Components and the Structure of Selenium Exopolysaccharide of Porphyridium sp.(Se-PSP)

作　　者：张威[1] 王敏[2] 朱劲华[1] 张成武[2] 

机构地区：[1]江苏职工医科大学,南京210029 [2]南京师范大学生命科学学院,南京210097

出　　处：《天然产物研究与开发》2006年第6期964-967,共4页Natural Product Research and Development

摘　　要：通过在ASW培养基中加入适量的亚硒酸制备硒化紫球藻胞外多糖,经分离纯化、纯度鉴定后,利用下列手段对其进行分析:通过紫外可见光谱扫描、红外光谱扫描了解其结构信息;通过高效液相色谱对其单糖组分进行分析;通过硫酸-咔唑法测定其糖醛酸含量;通过硫酸比浊法测定其硫酸根含量,等。Se-PSP和PSP一样,分离后分别得到两种成分,紫外光谱也和PSP相似,不含有蛋白质和核酸;红外光谱显示Se-PSP中Se可能取代了C-H上的H和SO42-中的S;HPLC显示其单糖组分种类相似,含量稍有差别;另外,PSP和Se-PSP所含的糖醛酸含量没有统计学差异,Se-PSP所含SO42-比PSP少。Se-PSP was purified by DEAE-52 cellulose column and identified by Sephadex G-200.The structure of Se-PSP was studied by UV spectrophotometer and IR spectrophotometer and its components was studied by HPLC, sulfuric acid-carbazole colorimetry and barium sulfate turbidimetry. The results showed that Se-PSP contained two members by DEAE-52 and Sephadex G-200 cellulose column. UV spectra of Se-PSP was similar to PSP,from which Se-PSP was proved to contain no protein and no nucleic acid. Se might replace H of C-H and S of SO4^2- by IR speetra. Se-PSP and PSP cotained the same kinds of and different quantity of monosaeeharides according to HPLC. The quantity of uronic acid had no difference between PSP and Se-PSP in statistics. The quantity of SO4^2- of Se-PSP was less than PSP.

关 键 词：硒 紫球藻 胞外多糖 

分 类 号：Q949[生物学—植物学]