可控性心肌缺血动物模型的制作  

Experimental animal model of controllable myocardial ischemia

作　　者：吴涛[1] 励建安[1] 陆晓[1] 黄澎[1] 邱峰[1] 孟祥博[1] 高晶[1] 

机构地区：[1]南京医科大学第一附属医院康复医学科

出　　处：《中国康复医学杂志》2006年第12期1068-1071,I0001,共5页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(30370687)

摘　　要：目的:建立小型猪可控性心肌缺血的动物模型。方法:健康成年小型猪10只,开胸安置自制水囊缩窄器,建立钝缘支可控性缺血的动物模型。术后行缺血心电图试验、冠脉造影、微球技术检测缺血区心肌水囊加压前后CCBF以及靶血管支配区心肌HE染色观察。结果:制作成功的8只小型猪在水囊加压后均可出现明显的缺血性心电图表现,同时钝缘支下游显影延迟、稀疏,钝缘支近乎完全闭塞,水囊压力撤除后心电图迅速恢复正常,钝缘支远端灌注恢复到水囊加压前状态。水囊加压前后靶血管支配区域CCBF明显减少(P<0.05)。HE染色无心肌梗死表现。结论:采用该自制水囊缩窄器可以成功制作可控性心肌缺血动物模型。Objective：To establish an animal model of controllable myocardial ischemia in piglet, Method：Thoracotomy and pericardiectomy were performed in 10 piglets. A home-made hydraulic occluder was then implanted around the oblique marginal artery. Selective coronary angiography,pressure-EKG test,CCBF and pathological examination were performed after surgery to evaluate the degree of stenosis.Result：Pressure induced EKG change of ischemia could be found in all subjects. Selective coronary angiography demonstrated a significant reduction in myocardial blood flow in the oblique marginal artery after hydraulic oceluder was inflated. Simuhaneously,CCBF of the hazardous zone was obviously decreased （P〈0.05）. There was no evidence of myocardial infarction.Conclusion：The home-made hydraulic oeeluder is applicable to make an animal model of controllable myocardial ischemia. It may create an important approach in research of cardiac rehabilitation.

关 键 词：可控性心肌缺血 动物模型 小型猪 

分 类 号：R-332[医药卫生]