联合转染p53与血管生成抑素基因对人胃癌细胞系SG7901生长的影响  

The effect of co-transfection of p53 and angiostatin gene in SG7901

作　　者：陈祥锦[1] 朱月永[2] 胡震霆[2] 张惠灏[1] 许东坡[1] 李明仁[1] 

机构地区：[1]福建医科大学附属第一医院肿瘤外科,福州350005 [2]福建医科大学附属第一医院肝病中心,福州350005

出　　处：《中国医师进修杂志》2006年第12期19-21,共3页Chinese Journal of Postgraduates of Medicine

基　　金：福建省自然科学基金资助项目(C0310017)

摘　　要：目的探讨联合转染p53和血管生成抑素(AS)基因对人胃癌细胞系SG7901生长的影响。方法用脂质体转染法分别将p53、AS基因、p53和AS基因转染人胃癌细胞系SG7901。以RT-PCR法检测转染后细胞目的基因的表达,通过细胞集落形成实验、MTT生长曲线、细胞周期检测观察其生物学特性变化。结果p53或AS基因均能抑制转染细胞的生长,而联合转染两种基因的细胞生长抑制更明显。结论p53和AS基因具有协同抑制恶性肿瘤细胞生长作用,提示联合多种基因可能有利于恶性肿瘤的基因治疗。Objective To investigate the co - transfection of p53 and angiostatin gene in the inhibition of SG7901. Methods Transfected the pVITR02 - hp53 - hAS into SG7901 with lipofectamine. After transfeetion, RT - PCR were used to know whether the aimed gene had been transfected and expressed or not. Cell clones trial, MTY growth curve, cell cycle measuring were used to analyze the differences. Results The cells were suppressed by the two genes and inhibition of the combined genes is more powerful than single one. Condtudon The effection of combined genes pVITR02 - hp53 - hAS on SG7901 is stronger than either single one. Combined - gene - therapy is a useful anti ,carcinoma method.

关 键 词：P53 血管生成抑素 基因治疗 恶性肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]