产尿激酶原CHO工程细胞无血清培基的研究  

Study on Serum-free Media for Genetically-engineered CHO Cells Producing Prourokinase

作　　者：陈昭烈[1] 吴本传[1] 贾熙华[1] 刘红[1] 肖成祖[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《生物工程学报》1996年第3期289-294,共6页Chinese Journal of Biotechnology

摘　　要：在分析产尿激酶原CHO工程细胞对培基中氨基酸和糖利用的基础上,对DMEM:F12(1:1)进行初步优化。采用正交实验设计建立了无血清培基11G-SG-SFM和11G-SE-SFM。用11G-SG-SFM悬浮培养11G细胞,细胞增殖速率与含5％小牛血清DMEM:F12或CHO-S-SFM相当。用11G-SE-SFM培养11G细胞,细胞增殖缓慢,但有利于提高11G细胞表达pro-UK的水平,pro-UK的表达水平比含5％小牛血清的DMEM:F12培养提高80％左右。Bsaed on the initial optimization of DMEM/F12 (1 : 1) , two kinds of serum-free media, 11G-SG-SFM and 11G-SG-SFM were formulated by using orthogonally designed experiments for the growth of genetically-engineered CHO cell line 11G and the expression of recombinant prourokinase in suspension culture, respectively. Growth of 11G cells in 11G-SG-SFM was comparable with that observed in DMEM/F12 (1 : 1) + 5% (v/v) NBS and CHO-S-SFM. 11G cells grew slowly in 11G-SE-SFM, but secreted relative high levels of recombinant prourikinase. The prourokinase productivity of 11G cells in 11G-S( ,-SFM reached 800-1000IU/106 - d and was about 80% and 20% higher than in DMEM/F12 + 5% NBS and CHO-S-SFM, respectively.

关 键 词：尿激酶原 CHO工程细胞 无血清培基 

分 类 号：TQ925.9[轻工技术与工程—发酵工程] Q81[生物学—生物工程]