新生儿输血传播病毒感染及基因序列特征  

DNA Sequence Features of Transfusion Transmitted Virus in Neonatal Infection

作　　者：周冬[1] 肖昕[1] 王文祥 

机构地区：[1]暨南大学附属第一医院新生儿科,广州510632 [2]中山市小榄医院儿科,广东中山528415

出　　处：《实用儿科临床杂志》2006年第24期1702-1703,1740,共3页Journal of Applied Clinical Pediatrics

基　　金：广东省医学科学技术研究基金项目资助(A2002897)

摘　　要：目的分析引起新生儿感染的输血传播病毒（TTV）ORF1区基因序列特征。方法应用巢式PCR技术检测外周血TTV-DNA，15例中国新生儿确诊为TTV感染；对新生儿感染的TTV（中国株，C01～C15）ORF1区进行基因序列测定。结果引起新生儿感染的中国株TTV与日本株N22比较，同源性达87．1％～97．7％，但存在着点突变，如112、113位点的GG→TT和236～240位点的TTATC→CCTAT。结论TTV中国株与日本株具有同源性，某些TTV基因突变可增强其致病性，可导致新生儿肝功能损害和结合胆红素升高。Objective To analyze the mutations in nucleotlde sequences of transfusion transmitted virus （TTV） in neonatal infection. Methods Neonatal serum TTV - DNA was detected by a nested PCR technique. Fifteen Chinese neonates with positive TTV - DNA were diagnosed as TTV infection, ORF1 sequences of TTV- DNA from these neonates were determined. Results Homology of Chinese TTV （C01 - C15） and Japanese TTV （N22）isolated ranged from 87. 1% - 97.7% at nueleotide level, but there were point mutations in Chinese TTV, such as GG→TT in locus 112 and 113, TTATC→CCTAT in locus 236 - 240. Conclusions Chinese and Japanese TTV isolated had the same genotype. Some gene mutations may increase the TTV pathogen,and result in neonatal hepatitis syndrome or hyperbilirubinemia.

关 键 词：输血传播病毒 感染 基因序列 婴儿 新生 

分 类 号：R722.1[医药卫生—儿科]