过钒酸钠对照射后BET-2细胞线粒体膜影响  

Effects of pervanadate on MMP of BET-2 cells induced by ionizing irradiation

作　　者：杨龙[1] 王奕萍[2] 荣曙[1] 许祥裕[1] 赵国良[1] 从玉文[3] 陈家佩[3] 

机构地区：[1]南京军区军事医学研究所博士后科研工作站,南京210002 [2]江苏省肿瘤医院B超室 [3]军事医学科学院放射医学研究所

出　　处：《中国公共卫生》2007年第1期62-63,共2页Chinese Journal of Public Health

基　　金：江苏省人事厅博士后基金项目(2004-100);江苏省六大人才高峰资助项目(2005-06)

摘　　要：目的研究酪氨酸磷酸酶抑制剂过钒酸钠(Pervanadate,Per)对电离辐射作用后细胞线粒体膜电位(ΔΨm)改变和细胞凋亡的影响。方法应用流式细胞仪测定电离辐射作用后鼠源性髓系白血病细胞株BET-2细胞线粒体膜电位及其在过钒酸钠干预后的改变和细胞凋亡水平。结果电离辐射早期可引起BET-2细胞线粒体膜电位降低,诱导细胞凋亡发生;过钒酸钠在一定程度上稳定细胞线粒体膜电位,减少细胞凋亡。结论过钒酸钠可逆转电离辐射诱导的细胞线粒体膜电位的降低,减少造血细胞凋亡的发生。Objective To study the effects of pervanadate（Per）, the inhibitor of protein tyrosine phosphotases on the alterations of mitochondrial membrane potential（MMP） and the apoptosis of BET - 2 cells induced by ionizing irradiation. Methods BET - 2 cells were divided into Per - treated groups and non - Per groups and incubated after ionizing irradiation. The MMP and apoptosis were measured with FCM. Results After irradiation was given, the MMP of BET- 2 cells was reduced in the early stage significantly, and the apoptosis appeared, and these changes had a dose - dependent pattern and a time - course fashion. Per potently enhanced the MMP of BET - 2 cells and prevented irradiation - treated cells from apoptosis. Conclusion Per reversed the repression of mitochondrial membrane potential and suppress apoptosis induced by ionizing irradiation in the hematopoietic cells.

关 键 词：BET-2细胞 电离辐射 线粒体跨膜电位(△ψm) 细胞凋亡 过钒酸钠 

分 类 号：R733[医药卫生—肿瘤]