转基因番木瓜及其深加工制品的PCR检测方法  

DETECTION OF GENETICALLY MODIFIED PAPAYA AND ITS HIGHLY-PROCESSED PRODUCTS BY PCR METHOD

作　　者：庄逸林[1] 相大鹏[2] 蔡颖[1] 陈燕勤[1] 梁希扬[1] 许业莉[1] 苏建晖[1] 

机构地区：[1]汕头出入境检验检疫局,广东汕头515041 [2]广东出入境检验检疫局,广东广州510623

出　　处：《食品研究与开发》2007年第1期126-129,共4页Food Research and Development

基　　金：广东检验检疫局科技立项(GDK08-2001)

摘　　要：对番木瓜不同材料及其深加工制品的核酸提取方法进行了优化,成功提取出DNA。选取番木瓜特异性的内源蛋白PAPAIN基因片段作为内参基因,用于检验提取DNA的质量,有效避免了聚合酶链式反应PCR的假阴性并能用于确定检测样品中是否含有番木瓜成分。选取国内目前应用比较多的外源目的基因环斑病毒外壳蛋白(CP)基因以及复制酶(RP)基因作为品系鉴定的基因。适用于出入境检验检疫部门和农业部门对转基因番木瓜及其制品的检验监测。This study optimized the DNA extraction methods for different materials of papaya and its highly-processed products,and extracted the DNA successfully, which is suitable for PCR reaction.Papain gene,a papaya species-specific protein gene was employed as internal control to access the DNA quality of the experimental system, which prevented pseudo-negative and the samples being papaya-derived.CP （PRSV Coat Protein） gene and RP （PRSV Replicase） gene were tested as identification genes.This method was convenient and credible for bureau of Entry-Exit inspection and bureau of agriculture to detect GM papaya and its processed products.

关 键 词：转基因 番木瓜 深加工制品 PCR检测 

分 类 号：TS255[轻工技术与工程—农产品加工及贮藏工程]