房水中细胞因子在兔外伤性白内障术后的动态变化(英文)  

Dynamic changes of cytokine in aqueous humor of rabbits after traumatic cataract

作　　者：唐松[1] 杜冰[1] 黄丽娜[1] 秦磊[1] 翁宏[1] 

机构地区：[1]暨南大学医学院附属深圳眼科中心深圳市眼科医院,广东省深圳市518001

出　　处：《中国组织工程研究与临床康复》2007年第2期383-386,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：深圳市科技局基金资助项目(200304194)~~

摘　　要：背景:细胞因子是一类多功能强效的细胞生长调节因子,调节多种细胞的生长过程。研究表明细胞因子作为炎症介质在眼部炎症反应中占据重要地位,但其在外伤性白内障术后的炎症反应中的动态变化目前研究较少。目的:探讨兔外伤性白内障囊外摘除术后房水中白细胞介素1、白细胞介素6及肿瘤坏死因子α表达水平的动态变化及其与眼内炎症反应的关系。设计:随机对照的动物实验。单位:暨南大学医学院附属深圳眼科中心深圳市眼科医院。材料:实验于2004-03/2006-03在暨南大学医学院附属深圳眼科中心研究所完成。选择健康新西兰成年家兔15只,30只眼。普通级,体质量2.5～3.0kg,雌雄不限(暨南大学第二临床医学院动物中心提供,许可证号:SYXK(粤)2005-0061)。实验前检查家兔双眼未见异常。将家兔随机分成3组,分别为正常对照组、外伤对照组及外伤手术组(行囊外晶体摘除术),每组5只,10只眼。白细胞介素1、白细胞介素6及肿瘤坏死因子α的检测试剂盒由深圳市雅为生物科技有限公司提供。方法:将外伤对照组和外伤手术组家兔全麻(静脉注射10%乌拉坦,1mL/kg)后,用5号针头自角膜缘穿刺进入前房,划破晶体前囊膜约5mm,制成双眼外伤性白内障动物模型。正常对照组家兔正常喂养。外伤对照组及外伤手术组家兔造模成功后双眼均滴用普通抗生素眼药水清洁结膜囊,3次/d。造模成功后第3天全麻(静脉注射10%乌拉坦,1mL/kg)外伤手术组的家兔,于手术显微镜下由制作动物模型的同一手术者施行常规双眼白内障囊外摘除术,进入前房后行水平截囊或利用外伤性前囊膜破口,进行水分离,娩出晶体核,冲洗干净晶体皮质,缝合切口。于手术当天及术后1d,3d,7d,14d分别检查外伤对照组和外伤手术组家兔前房炎症反应情况并分别抽取3组家兔的房水0.2mL做房水细胞计数及分类,同时通过双抗�BACKGROUND ： Cytokine is a poly-functional and effective regulator factor to regulate growth of multiple cells. Research- es suggest that, as an inflammatory medium, cytokines play a key role in inflammatory reaction of eye; however, there are rare studies on dynamic changes after traumatic cataract. OBJECTIVE： To investigate the relationship among inflammatory reaction and dynamic changes of interleukin 1 （IL-1）, IL-6 and tumor necrosis factor alpha （TNF-α） in aqueous humor of rabbits after extracapsular cataract extraction. DESTGN： Randomized controlled animal study SETTING ： Shenzhen Municipal Ophthalmology Hospital, Shenzhen Municipal Ophthalmology Center Affiliated to Medical College of Jinan University. MATERIALS ： The experiment was carried out in Institute of Shenzhen Municipal Ophthalmology Center Affiliated to Medical College of Jinan University from March 2004 to March 2006. Fifteen healthy adult New Zealand rabbits of 30 eyes, general grade, weighting 2.5-3.0 kg, of either gender, were provided by Animal Center of the Second Clinical Medical College of Jinan University [certification： SYXK （yue） 2005-006]. EyeS of rabbits were normal before experiment. All rabbits were randomly divided into normal control group, traumatic control group and operative group with 5 in each group and in total of 10 eyes in each group. IL-1, IL-6 and TNF-α kits were provided by Shenzhen Yawel Blotechnology Com; pany Limited. METHODS ： Rabbits in traumatic control group and operative group were totally anesthetized with intravenous injection of 10% 1 mL/kg urethan, and then, 5# needle was punctured from corneal Ilmbus to anterior chamber to scarify anterior membrane of lens about 5 mm to establish animal models of traumatic cataract of oculus uterque. Rabbits in normal control group were fed normally. After successful modeling, common antibiotic eyedrops was used to clean conjunctival sac of rabbits in traumatic control group and operative group 3 times a day. On the 3^rd day of success

关 键 词：白细胞介素1 白细胞介素6 肿瘤坏死因子 炎症 白内障 

分 类 号：R776.1[医药卫生—眼科]