依达拉奉对脑缺血再灌注后FADD、caspase-8表达的影响  

Effect of Edaravone on FADD and caspase-8 Protein Expression Following Focal Cerebral Ischemia/Reperfusion

作　　者：胡跃强[1] 肖波[1] 毕方方[1] 丁玲[1] 

机构地区：[1]中南大学湘雅医院神经内科,湖南长沙410008

出　　处：《中国神经免疫学和神经病学杂志》2007年第1期20-22,I0002,共4页Chinese Journal of Neuroimmunology and Neurology

基　　金：国家自然科学基金资助项目(30370514)

摘　　要：目的研究大鼠脑缺血再灌注后Fas相关死亡域蛋白(FADD)、caspase-8蛋白的表达及依达拉奉对其的影响。方法用免疫组化法测定缺血2 h后再灌注不同时相缺血半暗带FADD、caspase-8蛋白的表达。结果缺血半暗带皮质内FADD、caspase-8蛋白的表达于缺血再灌注后3 h明显上升,再灌注12 h达高峰(P<0.01),至再灌后24 h明显下降。依达拉奉能显著下调其表达(P<0.01,P<0.05)。结论脑缺血再灌注后缺血半暗带FADD、caspase-8蛋白的表达均明显增加,提示二者可能在脑缺血再灌注损伤中发挥重要作用,依达拉奉可能通过抑制其表达而达到脑保护的作用。Objective To study the protein expressions of Fas-associated with death domain protein （FADD） and caspase-8, and the effects of edaravone on them. Methods The rats were subjected to the middle cerebral artery occlusion for two hours, then killed at different time points after reperfusion. The protein expressions of FADD and caspase-8 were detected by immunohisto-chemistry. Results The protein expressions of FADD and caspase-8 were markedly increased at 3 h after reperfusion in the penumbra of rat cortex, and peaked at 12 h （P〈0.01）, then declined quickly at 24 h. Edaravone treatment could significantly downregulate the FADD and caspase-8 protein expressions after 3 h of reperfusion （P〈0. 01, P〈0.05）, Conclusions FADD and caspase-8 may play important roles in the cerebral ischemia and reperfusion injury. Edaravone could protect neurons from ischemic damage by decrease the expression levels of FADD and caspase-8.

关 键 词：脑缺血 FADD CASPASE-8 依达拉奉 

分 类 号：R734.1[医药卫生—肿瘤]