内吗啡肽-2的合成研究  

Synthesis of Endomorphin-2

作　　者：梁月洁[1] 孟庆国[1] 李明慧[1] 周莎莎[1] 

机构地区：[1]烟台大学药学院,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2007年第1期18-21,共4页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：烟台大学博士启动基金(YX04B18)

摘　　要：用液相多肽合成法合成具有镇痛作用的μ阿片受体的内源性配体———内吗啡肽-2.直接使用叔丁氧羰基保护的有关氨基酸,采用DCC/HOB t缩合法和混合酸酐法分别合成N-端二肽片段和C-端二肽片段,然后将N-端二肽片段和C-端二肽片段缩合成四肽,最后用50%的TFA/DCM脱除保护基,成功地合成了内吗啡肽-2.本实验方法与文献相比,步骤减少,后处理简便,收率提高.Endomorphin-2 （EM-2）, with the effect of opioid analgesia, has been proposed as the most potent endogenous ligand of the/x-opioid receptor. The synthesis of EM-2 is described by the liquid phase condensation. N-terminal dipeptide and C-terminal dipeptide have been synthesized directly with the method of mixed anhydride and DCC/HOBt condensation reaction, then coupled successfully, pure EM-2 is obtained. We have decreased the process and simplified the experiment as well as increased the yield of product.

关 键 词：液相合成方法 内吗啡肽-2 合成 缩合 混合酸酐法 

分 类 号：Q516[生物学—生物化学]