Bmp-2对牙周膜细胞增殖和碱性磷酸酶活性的影响  

Impact of recombinant human bone morphogenetic protein-2 on proliferation and alkaline phosphatase activity of human periodontal ligament ceils in vitro

作　　者：周泉[1] 吉建新[1] 廖伟娇[2] 邱志辉[3] 潘广祠[1] 张婷[2] 

机构地区：[1]广州医学院第一附属医院口腔科,广东广州510120 [2]广州医学院第一附属医院检验科,广东广州510120 [3]广州呼吸疾病研究所,广东广州510120

出　　处：《中国热带医学》2007年第1期10-11,20,共3页China Tropical Medicine

基　　金：广州市科技局资助项目(编号:1999-J-008-01)

摘　　要：目的探讨骨形成蛋白-2(BMP-2)对人牙周膜(PDL)细胞增殖及碱性磷酸酶(ALP)活性的影响。方法通过体外细胞培养技术,用噻唑盐(MTT)法检测BMP-2刺激前后人PDL细胞增殖的情况,用酶动力学方法检测ALP活性。结果BMP-2可明显促进PDL细胞增殖,在实验的5d范围内,浓度在25～400μg/ml时呈浓度-时间依赖关系,最佳效应浓度为200μg/ml(比对照组增加了193．4％),最佳效应时间均为3d。BMP-2可显著增强PDL细胞的ALP活性,25μg/L作用下ALP活性已经比对照组差异有显著性(P＜0．01),浓度为100μg/L时效果最佳(P＜0．001),从第2d起,能增强人PDL细胞的ALP活性,第5d时作用趋于缓和。结论BMP-2能促进人PDL细胞的分化和增殖,能促进细胞ALP的活性。Objective To evaluate biological effects in vitro of recombinant human bone morphoganetie protein - 2 on the proliferation and alkaline phosphatsse（ALP） activity of human periodontal ligament（PDL） cells. Methods PDL cells were cultured for 1,2,3,4,5 days in DMEM supplemented with 1% FBS along（contzol）, or containing BMP - 2（25,50,100,200 and 400μg/ml）. Proliferation of the PDL cells was measured by MTT colorimeteric assay. Alkaline phosphatase activity was determined by enzyme ki- netic method, Data was analyzed by ANOVA. Results BMP- 2 at concentration of 25 to 400μg/ml showed a significant effects. BMP - 2 promoted PDL cells growth in a dose - and time dependent manner. The mast optimal concentration of BMP- 2 was 200μg/ml （ P 〈 0.05）. BMP- 2 significantly increased ALP activity in PDL cells. Conclusion BMP - 2 can promote the proliferation and significantly increased ALP activity of PDL cells. The data suggest that BMP - 2 may be valuable in promoting the proliferation of periodontal connective tissue.

关 键 词：牙周膜细胞 骨形成蛋白-2 细胞增殖 碱性磷酸酶 

分 类 号：R781.4[医药卫生—口腔医学]