癌灶、癌旁组织和淋巴结转移灶DNA含量的对比分析  

Comparative study of cellular DNA content on cancer nests,para-cancer tissues and cancerometasis lymph nodes

作　　者：周振英[1] 张军妮[1] 朱月清[1] 宋心龙 林琳[1] 顾平[1] 

机构地区：[1]江苏省肿瘤防治研究所

出　　处：《齐鲁肿瘤杂志》1996年第4期256-258,共3页

摘　　要：作者用流式细胞术对５１例大肠癌和８６例乳腺癌患者的癌灶、癌旁组织和淋巴结转移灶细胞进行了ＤＮＡ含量和增殖活性（ＳＰＦ）的对比研究。结果表明，癌旁组织是否出现ＤＮＡ异倍体细胞，与癌灶关系不大。这提示，癌旁异倍体细胞群的出现可能是致癌因子的直接作用。在癌灶、癌旁和淋巴结对比研究时剔除各组的二倍体标本后，其异倍体病例间比较时发现癌灶ＤＩ值与淋巴结的十分接近（大肠癌分别为１．５６和１．５２）；乳腺癌则分别为１．７３和１．７０），癌旁组织则明显减低（分别为１．１９和１．５９）；其ＳＰＦ也显示了类似的变化规律。这表明，癌灶和淋巴结中癌细胞的生物学特性十分相似。这提示，淋巴结中的恶性细胞可能是直接从癌灶转移过来的，而癌旁组织的恶性细胞可能是新生的。DNA content and proliferative activation were measured on cancer nests, para-cancer tissues and cancerometasis Iymph nodes in 51 patients with colonic cancer and 86 patients with breast cancer by flow cytometry.The results showed that the appearance of aneuploid in para-cancer tissues does not depend on their cancer nests.The results demonstrated that the appearance of malignant cells in para-cancer tissues may be due to direct action of carcinogenisis factor.In comparative study on cancer nests,para-cancer tissues and cancerometasis lymph nodes after rejecting diploid tumors,we found that DI value was very similar between cancer neste and cancerometasis lymph nodes (the colonic cancer 1 56 and 1 52,the breast cancer 1.73 and 1.70 respectively).However DI value of para-cancer tissues were significantly decreasing (1.19 and 1.59 respectively).The SPE of these groups also appeared similar change.These findings suggest that biology characteristic were alike between the cancer nests and the cancerometasis lymph nodes.The results indicated that malignant cells in the cancerometasis lymph nodes were transfered from cancer nests,while malignant cells were a newborn cells nests in paracancer tissues.

关 键 词：流式细胞术 大肠癌 乳腺癌 DNA含量 增殖活性 

分 类 号：R730.2[医药卫生—肿瘤]