NAD(P)H氧化酶在血管紧张素Ⅱ损伤人胰岛细胞功能中的作用  

The role of NAD(P)H oxidase in human islet cell damage induced by angiotensin Ⅱ

作　　者：鲁辛[1] 蔡德鸿[1] 张桦[1] 孙嘉[1] 陈宏[1] 

机构地区：[1]南方医科大学珠江医院内分泌科,广州510282

出　　处：《广东医学》2007年第1期17-20,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30470829);广东省科技计划项目(编号:B30201)

摘　　要：目的探讨分离纯化的人胰岛细胞是否表达NAD(P)H氧化酶亚基p22phox,通过体外实验了解该酶在血管紧张素Ⅱ损伤胰岛细胞功能中所起的作用。方法对分离纯化后的胰岛分别进行p22phox亚基和胰岛素免疫荧光检测;使用化学发光法检测不同浓度血管紧张素Ⅱ、AT1受体拮抗剂及NAD(P)H氧化酶抑制剂干预下胰岛细胞胰岛素分泌功能。结果免疫荧光标记胰岛p22phox亚基和胰岛素均呈强阳性;血管紧张素Ⅱ抑制高糖刺激下胰岛细胞胰岛素的分泌,使用AT1受体拮抗剂或者NAD(P)H氧化酶抑制剂能拮抗血管紧张素Ⅱ的抑制作用。结论分离纯化的人胰岛细胞表达NAD(P)H氧化酶;血管紧张素Ⅱ可能通过激活细胞表面NAD(P)H氧化酶使胰岛细胞发生氧化应激,从而损伤胰岛细胞的分泌功能。Objective To investigate the existence of NAD（P） H oxidase compotent - p22phox in human isolated islets and to study the role of NAD（P） H oxidase in angiotensin Ⅱ induced damage of the isolated islet cell in vitro. Methotis The localization of p22phox and insulin in islets were investigated by immunoinfluroscent staining. Various concentra- tions of angiotensin Ⅱ, AT1 -receptor antagonist and NAD（P） H oxidase inhibitor（DP[） were applied for studying the insulin release of islets by means of cheraoluminescence. Results p22phox and insulin were strongly positively - stained, which means they were localized on isolated human pancreatic islets ; Exposure of the isolated islets to angiotensin Ⅱ induced a dose - dependent inhibition of glucose - stimulated insulin release. This inhibitory action was fully preventable by pretreatment of the islets with AT1 - receptor antagonist and DPI. Conclusion NAD （P） H oxidase exists in isolated human pancreatic islets. Angiotensin Ⅱ inhibits high - glucose - stimulated insulin serection by acitivating NAD（P） H oxidase and inducing oxidative stress.

关 键 词：人胰岛细胞 氧化应激 NAD(P)H氧化酶 血管紧张素Ⅱ 

分 类 号：R587.1[医药卫生—内分泌]