Ad-p27mt转染重组腺病毒治疗裸鼠内人胃癌的分子机制  

Molecular mechanism of recombinant adenovirus Ad-p27mt therapy for human gastric cancer in nude mice

作　　者：朱金水[1] 张强[1] 宋明全[1] 张卫平[1] 达炜[1] 陈金联[1] 孙群[1] 

机构地区：[1]上海交通大学附属第六人民医院消化科,上海市200233

出　　处：《世界华人消化杂志》2006年第36期3477-3481,共5页World Chinese Journal of Digestology

摘　　要：目的:研究Ad-p27mt转染重组腺病毒对人胃癌细胞凋亡的作用及机制.方法:Ad-p27mt转染重组腺病毒导入胃癌细胞株SGC-7901内;流式细胞仪检测凋亡染色体亚二倍体峰值,了解Ad-p27mt对人胃癌组织凋亡的作用;TUNEL法检测DNA片断,在Ad-p27mt组和Ad-LacZ组中分析细胞的凋亡.结果:Ad-p27mt成功转入人胃癌细胞SGC-7901内,转化率达100%.流式细胞仪检测发现在感染后18h出现G1-S相前出现凋亡染色体亚二倍体峰值,并且DNA电泳出现凋亡特征性的条带;TUNEL法检测Ad-p27治疗组与对照组的凋亡率分别是92.3%±3.76%和2.01%±0.15%,两组的差异有显著性(P<0.01).结论:重组腺病毒转染的人p27突变基因能诱导裸鼠体内人胃癌细胞SGC-7901的凋亡.AIM： To explore the effect of recombinant adenovirus Ad-p27mt on the apoptosis of human gastric cancer cells and its correlative mechanism. METHODS： The recombinant adenovirus Ad- p27mt was constructed and then transfected into human gastric cancer cell line SGC-7901. The apoptofic peak of hypodiploid chromosomes in SGC-7901 cells was measured by flow cytometry, and the apoptosis of SGC-7901 cells was detected by TUNEL method. RESULTS： Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18-h infection, the apoptotic peak of hypodiploids appeared before G1-S phase and the bands with apoptotic characteristics were shown by DNA electrophoresis. The apoptotic rate of SGC-7901 cells detected by TUNEL method was 89.4%±3.12% （Ad-p27mt group） and 3.12%±0.13% in Ad-p27mt-transfected cells and controls, respectively, and there existed signifi-cant difference between the two groups （P〈0.01） . CONCLUSION： Human mutant p27 can induce the apoptosis of human gastric cancer cells in vitro.

关 键 词：胃癌 人突变基因p27 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]