小麦基础饲料添加木聚糖酶对尼罗罗非鱼肠道食糜粘度和绒毛、微绒毛发育的影响  

The influences of xylanase added in wheat basal diet on intestine chyme viscosity and the development of villi and microvilli of Tilapia nilotica

作　　者：聂国兴[1] 王俊丽[1] 朱命炜[1] 周洪琪[2] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]上海水产大学生命科学与技术学院,上海200090

出　　处：《水产学报》2007年第1期54-61,共8页Journal of Fisheries of China

基　　金：河南省重点科技攻关项目(0423014000);河南省动物学重点学科资助项目

摘　　要：以尼罗罗非鱼[体重(106.16±16.77)g]为实验对象,小麦基础饲料为对照,小麦基础饲料中分别添加不同水平的木聚糖酶(0.05%、0.10%、0.15%)作为试验饲料。每个处理设5个重复,每个重复放养40尾雄性尼罗罗非鱼。饱食投喂,饲养75d后测定尼罗罗非鱼肠道食糜粘度以及前、中、后肠的肠绒毛显微与超微结构。结果表明,对照组与0.05%、0.10%、0.15%木聚糖酶试验组的肠道食糜粘度分别为(15.21±0.29)cps、(13.75±0.45)cps、(12.23±0.16)cps和(11.92±0.22)cps,试验组的肠道食糜粘度极显著低于对照组(P<0.01)。随着木聚糖酶添加量的增加,试验组尼罗罗非鱼前、中、后肠绒毛表面的粘附性颗粒数量较对照组明显减少,颗粒直径减小;试验组前、中、后肠微绒毛的密度和高度均得到不同程度的改善。0.10%木聚糖酶试验组前、中、后肠绒毛高度、宽度、密度均极显著高于对照组(P<0.01)。Wheat is important cereal crop in our country. Recently, because of the rise of science and technology, the output of wheat have increased significantly and exceeded in phase and structure. Meanwhile, the flourishing developments of aquatic feed industries have been restricted seriously by the defect of raw material. Using wheat as raw material of feed, carrying out transformation and consumption by aquaculture, all of these now possess the resources feasibility. The use of wheat in aquatic feed industry as major energy feed has been restricted because of xylan. In the past few years, the application of feed xylanase with wheat basal diet has resolved the problems of the anti-nutrition of xylan successfully.Xylanase could improve the growth of aquatic animals fed with wheat basal diet. But there weren＇ t any researches about the mechanism of xylanase promoting aquatic animals＇ growth at home and abroad. The purpose of adding xylanase to wheat basal diet in this paper was to study the influences of xylanase on intestine chyme viscosity, on the developments of intestine villi and microvilli. In this study we also probed into the relationship between intestinal environment and the developments of villi and microvilli, displayed the mechanism of xylanase relieving the anti-nutrition of xylan and promoting Tilapia nilotica＇ s growth. Wheat basal diet was control. The tested diets were wheat basal diet added with different levels of xylanase （0.05%, 0.10%, and 0.15% respectively）. Each treatment was devised with 5 repeats and each repeat had 40 male Tilapia nilotica. The fish in floating cages were fed to satiation for 75 days. The results indicated that adding xylanase to wheat basal diet could decrease intestine chyme viscosity, promote the developments of villi and microvilli of Tilapia nilotica. The intestine chyme viscosity of 0.05% ,0.10%, 0.15% xylanase groups [ （ 13.75 ±0.45） cps, （ 12.23 ±0.16） cps and （ 11.92 ±0.22） cps respectively） ] was significantly lower than that of control [

关 键 词：小麦基础饲料 木聚糖酶 尼罗罗非鱼 食糜粘度 肠绒毛 

分 类 号：S963[农业科学—水产养殖]